犬瘟热病毒Guizhou株血凝素基因的克隆及序列分析  

Cloning and Sequence Analysis of Haemagglutinin Gene of Guizhou Strain of Canine Distemper Virus

作　　者：朱军莉[1] 余旭平[1] 张登祥[2] 沈琴芳[1] 徐在平[2] 李永明[2] 周碧君[2] 吴彤[2] 

机构地区：[1]浙江大学动物科学学院 [2]贵州大学动物科学学院,贵州贵阳550025

出　　处：《中国兽医学报》2006年第1期17-20,共4页Chinese Journal of Veterinary Science

基　　金：贵州省科技攻关资助项目[(2000)111]

摘　　要：参照已发表的文献合成1对引物，以犬瘟热病毒Guizhou分离株感染Vero细胞收获病毒提取的RNA为模板，RT-PCR扩增获得血凝素蛋白基因（H）901～1661位大小为761bp的片段，并将PCR扩增产物进行克隆和测序。将Guizhou株与GenBank数据库中的31株CDV及国内NanjingOP株的H基因序列进行同源性和系统发生树分析，发现Guizhou株的H基因部分序列与疫苗毒株Convac和Onderstpoort的同源性最低，只有90％；与日本野毒株Tanu96、KDK-1、Hmanatsu、Yanaka、UENO的同源性较高，为96％～98％；且与国内从大熊猫和小熊猫分离的野毒株GP和LP的同源性也较高，为98％和96％。系统发生树分析显示，Guizhou株与国内野毒株GP和LP，及日本的5个野毒株应属同一类，其中和GP株基因型最近，推测这些毒株间有更近的共同祖先。因此怀疑该病毒较适应于野生动物，且在我国某些地区的野生动物中可能存在自然疫源性传播。Using a pair of primers and RNA template extracted from Vero cells infected with CDV Guizhou strain,a 761 bp fragment between 901 bp and 1 661 bp of Haemagglutinin protein gene（H） was amplified by RT-PCR. The PCR product was cloned and then sequenced. The homology between the H genes of Guizhou strain and 31 CDV strains in GenBank database and a published Chinese strain Nanjing OP.unsubmitted was analyzed and a phylogenetic tree was built. The result showed that the Guizhou strain had the lowest homology（only 90% ） with vaccine strains of Onderstepoort and Convac. However it had 96%- 98 % homology with Japan strain Tanu 96, K DK- 1, Hmanatsu, Yanaka, UENO isolated in late 1990＇s ,and had 98 % and 96 % homology with chinese strains GP and LP ,which were isolated from panda and lesser panda .respectively. Phylogenetic analysis indicated that Guizhou strain was clustered with GP and LP and 5 Japan strains of late 1990＇s.in which the GP strain was the nearest one. Thus it inferred that they might have common ancestor and were easy to adapt to wild animals and widely spread in China.

关 键 词：犬瘟热病毒 H基因 序列分析 同源性 

分 类 号：Q78[生物学—分子生物学] S852.65[农业科学—基础兽医学]