CD2AP、F-actin在肾病大鼠中的表达及意义  

Expression of CD2AP and F-actin in rats with puromycin aminonucleoside nephrosis

作　　者：赵非[1] 黄松明[1] 张爱华[1] 费莉[2] 郭梅[2] 陈荣华[2] 

机构地区：[1]南京医科大学附属南京儿童医院,江苏南京210008 [2]南京医科大学儿科研究所,江苏南京210029

出　　处：《中国病理生理杂志》2006年第1期182-186,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助(No.30100081);南京医科大学科研基金资助(No.NY02039)

摘　　要：目的:观察CD2-associatedprotein(CD2AP)、肌动蛋白微丝(F-actin)在氨基核苷肾病大鼠肾小球中的表达变化及其意义。方法:通过建立大鼠氨基核苷肾病模型,采用免疫组织化学、蛋白质印迹技术观察CD2AP在不同时点肾病大鼠肾小球中的表达和分布变化,采用荧光技术检测肾小球F-actin含量的变化。结果:①肾小球足细胞CD2AP的表达在肾病模型建立的早期即有下调;在肾病大鼠蛋白尿的高峰期,CD2AP的表达明显下降(P<0.01);在肾病大鼠的疾病恢复期,CD2AP的表达逐步恢复正常。②肾小球足细胞CD2AP表达量的变化与肾病大鼠24h尿蛋白的变化呈负相关(r=-0.865,P<0.01)。③在肾病大鼠蛋白尿的高峰期,肾小球F-actin含量明显下降(P<0.05)。④肾小球CD2AP蛋白表达量和肾小球F-actin荧光定量变化呈正相关(r=0.873,P<0.01)。结论:①CD2AP、F-actin的表达变化在蛋白尿的发生机制中有着重要作用。②CD2AP可能是判断肾小球足细胞损伤的一个早期重要指标。AIM： To investigate the role of CD2AP and F- actin in the pathogenesis of puromycin aminonucleoside nephrosis in rats. METHODS： Puromycin aminonucleoside nephrosis was induced by single intrapefitoneal injection of puromycin aminonucleoside （PAN）. Renal tissues were studied at 3, 7, 10 and 20 days after PAN injection by means of immunohistochemistry, RT- PCR, Western blotting and fluorescence. RESULTS： At day 3, CD2AP expression in podocytes began to decrease, and significantly decreased at day 7 and 10 （ P 〈 0.01 ）. These occurred in association with an increase in 24 h urinary protein content （ r = - 0. 865, P 〈 0.01 ）. At day 10, a remarkable decrease in F - actin was found in the glomeruli of PAN rats （ P 〈 0.05）. Positive correlation was found between CD2AP and F-actin expression （ r = 0.873, P 〈 0.01）. CONCLUSION： CD2AP expression may be a useful marker of acute injury in podocytes. CD2AP and F- actin are also likely to be involved in the pathogenesis of proteinuria.

关 键 词：CD2-associated PROTEIN 肾病 细胞骨架 微丝 

分 类 号：R363[医药卫生—病理学]