涎腺肿瘤survivin的免疫组织化学表达及其意义  

The immunohistological expression of survivin in salivary gland neoplasms

作　　者：梁永强[1] 徐江 张彬[1] 齐红[3] 白宇宏[1] 孙树勋[4] 

机构地区：[1]华北煤炭医学院口腔系,唐山063000 [2]铜川市人民医院口腔科 [3]西安交通大学口腔医学院病理科 [4]华北煤炭医学院基础部

出　　处：《现代口腔医学杂志》2006年第1期52-56,共5页Journal of Modern Stomatology

摘　　要：目的通过检测涎腺肿瘤中survivin蛋白的表达情况,探讨survivin在涎腺肿瘤发生发展中的作用。并通过常规切片与组织芯片的对比研究,探讨组织芯片在涎腺肿瘤研究中应用的可行性。方法收集涎腺肿瘤标本115例。制备普通切片及组织芯片。用免疫组化方法显示普通切片和组织芯片中的survivin,显微图象分析软件测定平均光密度值,对数据进行统计分析。结果survivin在正常涎腺组织中不表达,在良性肿瘤中仅在多形性腺瘤中表达,涎腺恶性肿瘤中均有不同程度表达。涎腺癌高分化组和中低分化组及多形性腺瘤组MOD值分别为0.20±0.029,0.38±0.052,0.18±0.021。涎腺癌survivin表达与淋巴结转移及复发有相关性。制备的涎腺肿瘤组织芯片形成124点阵的组织阵列。常规切片与组织芯片survivin免疫组化结果比较有较好的一致性。结论survivin表达水平,大致能反映涎腺癌的恶性程度。survivin可能通过抑制涎腺癌的细胞凋亡,对涎腺癌的发生发展起重要作用;组织芯片与常规切片免疫组化染色结果有良好的一致性。组织芯片将会成为涎腺肿瘤研究中一个经济、高效的方法和工具。Objective To detect the expression of survivin in salivary gland neoplasms, to analyse the significance of survivin in the occurance and the development of salivary gland neoplasms, and to analyze the possibility of the application of the tissue chip in the study of salivary gland neoplasms by comparing the tissue chip and regular sections. Methods One hundred and fifteen cases of salivary gland neoplasm were collected. The regular sections and the tissue chip were obtained. The survivin protein of the regular sections and tissue chip were determined using the immunohistochemistry method. The mean optic density was measured in the image analyse system and the data was analysed with the SPSS software. Results Survivin did not expressed in the normal salivary gland tissue. Most of the malignant salivary gland neoplasms became expressed with survivin. The survivin expression was only observed in pleomorphic adenoma in benign salivary gland neoplasms. The MOD were 0.20±0.029, 0.38±0.052, and 0.18±0. 021 in the high differentiation group, low differentiation group, and PA group, respectively. The survivin expression was correlated with the lymph node metastasis and with the recurrance in salivary gland carcinoma. Among the 124 spots of tissue array made up, the immunohistochemesty results of regular sections were consistent with that of tissue chip. Conclusion The expression level of survivin perhaps reflects the malignant degree of salivary gland carcinoma. Survivin possibly plays an important role in the occurrence and the development of salivary gland carcinoma. The immunohistochemesty results are cohered between the regular sections and tissue chip. Tissue chip may become an economical and useful tool in the study of salivary gland neoplasms.

关 键 词：SURVIVIN 涎腺肿瘤 组织芯片 免疫组化 口腔病理 

分 类 号：R739.8[医药卫生—肿瘤]