端粒酶启动子肿瘤靶向TNF相关凋亡诱导配体基因抗大肠癌细胞HT-29的活性研究  

Activity of the TNF-related apoptosis-inducing ligand gene expressed from the hTERT promoter on colon cancer cell line HT-29

作　　者：陈斌[1] 何超[1] 劳伟峰[1] 黄学锋[1] 方炳良[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院 [2]美国MD Anderson癌症中心

出　　处：《浙江大学学报（医学版）》2006年第1期45-49,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(30271467)

摘　　要：目的:探讨在端粒酶(hTERT)启动子驱动下TRA IL基因在大肠癌细胞HT-29的表达及其杀细胞作用。方法:通过腺病毒载体系统将hTERT启动子驱动的TNF相关凋亡诱导配体(TRA IL)基因转入大肠癌细胞HT-29,流式细胞仪检测GFP/TRA IL的表达和HT-29细胞凋亡率。结果:端粒酶启动子驱动的GFP/TRA IL基因和CMV启动子驱动的GFP(绿色荧光蛋白)基因在HT-29内的表达率分别达31.4%和67.0%;GFP/TRA IL基因对HT-29细胞的生长抑制率和凋亡率分别达74.2%和25.8%,与PBS和A d/CMV-GFP比差异都有显著性意义(P<0.05)。结论:端粒酶启动子驱动的GFP/TRA IL融合基因能在大肠癌细胞中有效表达;TRA IL基因对大肠癌细胞HT-29有明显的抑制生长和促凋亡作用。Objective： To evaluate the expression and activity of TNF-related apoptosisinducing ligand （TRAIL） gene expressed from the hTERT promoter on colon cancer cell line HT-29. Methods： GFP/TRAIL gene expressed from the hTERT promoter was transfected into HT-29 with adenoviral vectors system,expression and apoptosis inducing ability of GFP/TRAIL protein were determined with fluorescence-activated expression of GFP gene was 31.4% and 67.0% with in DLD1 cells; GFP/TRAIL gene was able to （25.8%）of HT-29 cells. There was significant cell sorting （FACS） method. Results： The either hTERT promoter or CMV promoter inhibit cell growth（74.2%） and induce apoptosis difference between Ad/hTERT-gTRAIL and the other two control groups （PBS and Ad/CMV-GFP,P〈0. 05）. gene with hTERT promoter transfected by adenoviral vector was Conclusion： The GFP/TRAIL successfully expressed in HT-29 cell,which can both inhibit cell growth and induce apoptosis of colon cancer cell line HT-29.

关 键 词：肿瘤坏死因子/分析 端粒 末端转移酶/遗传学 肿瘤细胞 培养的 蛋白质类/化学 结肠直肠肿瘤/病理学 基因疗法 细胞凋亡 

分 类 号：R735.34[医药卫生—肿瘤]