骨髓基质干细胞在肌营养不良模型鼠中的分布及移植途径对分布的影响  

In vivo distribution of bone marrow-derived mesenchymal stem cells after infusion and the influence of different infusion portals

作　　者：李经伦[1] 冯善伟[2] 柳太云[2] 于美娟[2] 张成[2] 刘长征[1] 

机构地区：[1]中山大学基础医学院实验核医学科 [2]中山大学第一附属医院神经内科

出　　处：《中华神经科杂志》2006年第1期32-35,共4页Chinese Journal of Neurology

基　　金：国家自然科学基金资助项目(30370510)

摘　　要：目的研究干细胞在mdx鼠体内较长时间的动态分布以及移植途径对它的影响。方法用3H-TdR掺入标记骨髓基质干细胞,用4×106个/只静脉及腹腔移植放疗mdx鼠各30只,在24h、48h、2周、1个月、2个月、4个月时间点处死小鼠,取血、心、肝、脾、肺、肾、肌、脑、骨髓消化测定放射计数,计算%ID/g值。结果①静脉移植的mdx鼠脑、骨髓在2周才达到它自身的最高水平,心肌、骨骼肌4个月时达到高峰;而每个时间点各器官含量相比早期基本是肺、肝、骨髓分布多,心肌、肌肉2个月后仅次于骨髓。②腹腔移植mdx鼠血、肺、肾、肝、脾脏、脑到达它各自的高峰比静脉注射均有不同时间延迟,早期还是在骨髓、肝、肺分布多,肌肉2个月后仅次于骨髓。③心、肌肉、脑、骨髓含量静脉移植比腹腔移植高,均具有显著的统计学意义(P<0.01)。结论干细胞移植mdx鼠后4个月达高峰,移植途径对干细胞的分布有影响,3H-TdR能长时间动态观察干细胞分布。Objective To investigate the dynamic distribution of bone marrow-derived mesenchymal stem cells after infusion for a longer time and the influence of different infusion portals. Methods Bone marrow-derived mesenchymal stem cells labeled with 3 H-TdR were injected into 30 irradiated mdx mice via tail vein or intraperitoneal cavity, in a quantity of 4 × 10^6 per mice. The radioactivity concentration of blood, heart, liver, spleen, lung, kidney, muscle, brain and bone marrow were detected at 24-hour, 48-hour, 2-week, 1-month, 2-month, 4-month points after infusion and calculated by % ID/g with liquid scintillation counting. Results ① Radioactivity concentration of brain and bone marrow reached the highest level at 2-week time point after infusion via tail vein, but that of heart and muscle did at 4-month point. At early time, the stem cells mainly distributed in lung, liver and bone marrow, then at 2-month point the distribution in muscle and heart muscle was increased but still less than that in bone marrow.② The time for radioactivity concentration of blood, lung, kidney, spleen, brain to reach the peak was retarded via peritoneal cavity than that via tail vein. At early time, the concentration in lung, liver and bone marrow was similar to that via tail vein, the concentration of muscle was lower than that of the bone marrow at 2-month point. ③Radioactivity concentration of heart, muscle, brain, bone marrow was much higher via tail vein compared to that via peritoneal cavity, showing a statistical significance （P 〈 0. 01 ）. Conclusions Radioactivity concentration in muscle reaches the highest level at 4-month point after infusion and is influenced by different infusion portals. 3^H-TdR should be an excellent tracer for observing the dynamic distribution of stem cells for a longer time.

关 键 词：肌营养不良 间质干细胞移植 骨髓细胞 

分 类 号：R746[医药卫生—神经病学与精神病学]