高糖时细胞间相互作用对共培养ECV304细胞的影响及茶多酚的干预作用  

Effect of glomerular intercellular interaction under high glucose concentration on production of ROS and TGF-β_1 in co-cultured ECV304 cells and intervention with tea polyphenols

作　　者：邓红[1] 李海浪[2] 王凯[2] 曾玉[3] 刘必成[4] 

机构地区：[1]浙江大学医学院环境基因组中心病理学与病理生理学系,浙江杭州310009 [2]东南大学附属中大医院儿科,江苏南京210009 [3]东南大学基础医学院病理学与病理生理学系,江苏南京210009 [4]东南大学附属中大医院肾内科,江苏南京210009

出　　处：《中国病理生理杂志》2006年第2期326-329,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370658)

摘　　要：目的:研究高糖时系膜细胞对体外共培养的ECV304细胞产生活性氧和转化生长因子β1(TGF-β1)的影响以及茶多酚的干预作用。方法:建立体外ECV304细胞和系膜细胞不直接接触共培养体系,分为正常对照组、高糖组、茶多酚组、茶多酚干预组,并与两种细胞分别单独培养进行比较,培养36h后,分光光度比色法测定细胞上清液超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,RT-PCR法测定培养ECV304细胞内TGF-β1mRNA的表达。结果:在共培养模型中,高糖抑制SOD活性,促进MDA产生,上调共培养模型中ECV304细胞TGF-β1mRNA表达,其作用显著高于单独培养的ECV304细胞,茶多酚干预可拮抗高糖时ECV304细胞的上述改变。结论:(1)高糖环境下系膜细胞和ECV304细胞间存在相互作用,这种作用能促进共培养的ECV304细胞产生活性氧和上调TGF-β1的表达。(2)茶多酚通过干预这种细胞间相互作用,保护ECV304细胞。AIM： To investigate the effect of glomerular intercellular interaction under high glucose concentration on the production of reactive oxygen species （ROS） and transforming growth factor β1 （TGF- β1 ） in co- cultured human ECV304 cells, and to study the intervention with tea polyphenols （TPs）. METHODS： The endothelial cells were cultured alone or co-cultured with mesangial cells in high glucose media with or without TPs for 0 h, 12 h and 36 h, respectively. The activity of SOD and the content of MDA in the media of the system were detected by spectrophotometry. The expression of TGF - β1 mRNA in the endothelial cells was measured by using semi- quantitative reverse transcription PCR （RT- PCR）. RESULTS： High glucose decreased the activity of SOD, increased the content of MDA and up - regulated the expression of TGF - β1 mRNA in co - cultured ECV304 cells and the effect became more prominent than the single- cultured cells. TPs interrupted it more effectively. CONCLUSION： These data suggest that there is interaction between mesangial cells and ECV304 cells under high glucose concentration. The interaction may markedly up - regulate the production of ROS and the expression of TGF - β1 in co - cultured ECV304 cells. TPs may protect ECV304 cells by intervening intercellular interaction.

关 键 词：共同培养 葡萄糖 茶多酚 活性氧 转化生长因子Β ECV304细胞 

分 类 号：R363[医药卫生—病理学]