感光细胞缺失对视网膜节细胞melanopsin表达的影响  

Effects of photoreceptor degeneration on expression of melanopsin in retinal ganglion cells

作　　者：万瑾[1] 郑华[1] 韩秀引[1] 肖虹蕾[1] 佘振珏[1] 周国民[1] 

机构地区：[1]复旦大学上海医学院解剖与组织胚胎学系,上海200032

出　　处：《解剖学杂志》2006年第1期18-21,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30471846)教育部重大重点项目(102096)

摘　　要：目的：研究感光细胞缺失对视网膜节细胞 melanopsin 表达的影响。方法：SD 大鼠腹腔注射60 mg/kg N- 甲基-N-亚硝基脲建立感光细胞变性缺失的动物模型,用免疫荧光方法观察视网膜铺片及切片中 melanopsin 阳性细胞的分布及特征。结果：在药物注射12 h 后动物视网膜感光细胞层开始凋亡,细胞数目随时相减少,13 d 后完全消失。在正视网膜节细胞表达 melanopsin,呈串珠样分布在胞体及突起的胞膜上,其树突穿过节细胞层, 在内网层的两侧分叉形成网状结构。在感光细胞缺失动物模型组,12 h 时,节细胞表达 melanopsin 更广泛；而后突起上的 melanopsin 表达随时减少。28 d 时,melanopsin 的表达主要局限于节细胞的胞体。结论：感光细胞缺失影响节细胞突起 melanopsin 的表达。Objective. To investigate the effects of photoreceptor degeneration on the expression of melanopsin. Methods： Photoreceptor degenerative model was made by i. p injection of N-methyl-N-nitrosourea at a dose of 60 mg/kg. Melanopsin positive cells were observed by fluorescent immunohistochemistry on whole-mount and eye sections. Results： Apoptosis of photoreceptors appeared 12 h after injection; photoreceptors decreased as time went by, and disappeared on 13 d. Melanopsin expressed on ganglion cells. The stained processes had a beaded appearance; dendrites bifurcated at the outermost and innermost of inner plexiform layer （ipl）, forming reticular network. Melanopsin was extensively expressed 12 h after injection, then melanopsin expression in dendrites decreased gradually; on 28 d melanopsin expression was confined to cell bodies. Conclusion： Photoreceptor degeneration affects melanopsin expression on ganglion cells dendrites.

关 键 词：MELANOPSIN 视网膜神经节细胞 感光细胞 

分 类 号：R774.1[医药卫生—眼科]