凝胶净化液相色谱法同时检测染红食品中对位红和苏丹红染料  

SIMULTANEOUS HPLC DETERMINATION OF PARA-RED AND SUDAN RED DYESTUFFS IN FOODSTUFFS AFTER PURIFICATION WITH GEL PERMEATION CHROMATOGRAPHY

作　　者：骆和东[1] 贾玉珠[1] 朱宝平[1] 林健[1] 

机构地区：[1]厦门市疾病预防控制中心,厦门361004

出　　处：《理化检验（化学分册）》2006年第2期86-90,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：研究了采用凝胶色谱法净化，HPLC-DAD同时快速测定食品中对位红和苏丹红Ⅰ-Ⅳ号染料的方法。样品经环已烷／乙酸乙酯（1＋1）萃取，Bio-Beads SX3凝胶层析柱净化去除油脂、天然色素等大分子干扰物质。采用Zorbax SB-C18（4．6mm×250mm，5μm）色谱柱，以乙腈-乙酸水溶液（pH4．0）为流动相梯度洗脱，二极管阵列检测器多波长同时检测染红食品中对位红和苏丹红Ⅰ-Ⅳ号染料。在0．1～10．0mg·L^-1浓度范围内，方法具有良好的线性关系（r〉0．999），样品的平均回收率为88．6％～99．2％，相对标准偏差为1．35％～3．57％，对位红和苏丹红Ⅰ-Ⅳ的检出限分别为1．8，5．0，9．5，8．0，6．5μg·kg^-1。A study on the simultaneous determination of para red and sudan red dyes （in cluding sudan Ⅰ, Ⅱ, Ⅲ and Ⅳ） by HPLC with diode array detector after purification with gel permeation chromatography was reported in this paper. Samples were extracted directly with a mixture of cyclohexane-athylaeetate （1 ＋ 1） and purified by passing through a column packed with No-beads SX3 gel, to remove interfering high molecular fatty substances and natural colorants in food samples. Chromatographic column of Zorbax SB C18 （4. 6 mm× 250 mm, 5 μm） was applied, and aqueous solution of acetonitrile, containing 3 drops of acetic acid （pH 4. 0） was used as mobile phase in the gradient elution. Multiwavelength diode-array detection was applied in the determination of the 5 compounds mentioned above. Linear relationships in the range from 0. 1 to 10 mg · L^- l of either of the 5 compounds were obtained with correlation coefficients （r） larger than 0. 999. RSD＇s obtained were in the range of 1.35%0 to 3.57%, and average recoveries were in the range of 88. 6% to 99. 2%. Detection limits were found to be 1.8 μg ·kg^-1, 5.0μg ·kg^-1, 9. 5μg ·kg^-1 , 8. 0 μg ·kg^-1 and 6. 5 μg ·kg^-1 for para red, sudan Ⅰ, sudan Ⅱ, sudan Ⅲ and sudan Ⅳ respectively.

关 键 词：凝胶净化 液相色谱法 对位红 苏丹红 食品 

分 类 号：O657.37[理学—分析化学]