纤维素酶法提取胡芦巴甾体总皂苷工艺条件的优化  

Studies on Extraction Technology of Total Steroidal Saponins from Fenugreek Seeds by Cellulase

作　　者：张黎明[1] 李霞[1] 徐玮[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《食品科学》2006年第2期157-160,共4页Food Science

基　　金：天津市高等学校科技发展基金资助项目(20031210)

摘　　要：以甾体皂苷元的提取率为指标,应用L9(34)正交试验设计优化了胡芦巴种子中甾体皂苷的酶法提取工艺,实验结果表明,影响酶法提取甾体皂苷的主次因素为:酶用量>提取温度>固液比>水解时间。纤维素酶处理能显著提高甾体皂苷的提取率,加酶后甾体皂苷元提取率的平均值为83.6%,而未加酶的结果则为58.4%。酶法提取新工艺能显著提高胡芦巴甾体皂苷的提取率。Using enzyme-extracting methods, the optimum extraction conditions of steroidal saponins from the of fenugreek seed flour were investigated by orthogonal design with the extracting rate of steroidal sapogenin as index. The results showed that the factors affecting extraction efficiency of steroidal saponins were as follows： the enzyme dosage 〉 treatment temperature 〉 ratio of solid to liquid （g/ml） 〉 treatment time. The percentage of extraction of steroidal sapogenin could be obviously raised by enzymic treatment, the average value was 83.6% for enzyme-extracting method and 58.4% for the same condition without cellulase. The extracting percentage of steroidal saponins from fenugreek seeds could be improved by cellulase treatment.

关 键 词：胡芦巴 甾体皂苷 酶处理 提取工艺 

分 类 号：R284.2[医药卫生—中药学]