单宁酶和β-葡萄糖苷酶的共固定化  

Studies on the Co-immobilization of Tannase and β-glucosidase

作　　者：苏二正[1] 夏涛[1] 宛晓春[1] 张正竹[1] 黄继轸[1] 

机构地区：[1]安徽农业大学农业部茶叶生物化学与生物技术重点开放实验室,安徽合肥230036

出　　处：《食品与生物技术学报》2006年第1期40-44,共5页Journal of Food Science and Biotechnology

基　　金：教育部重点科研课题项目(02067);安徽省优秀青年科技基金资助项目

摘　　要：比较了海藻酸钠和壳聚糖两种载体及不同固定化方法对单宁酶和β-葡萄糖苷酶的共固定化效果,结果表明以海藻酸钠为载体,采用交联-包埋-交联固定化方法的效果最佳。对共固定化条件进行了优化,可使单宁酶和β-葡萄糖苷酶的活力回收率分别达67.3%和46.0%。The effects of two kinds of carriers and four different kinds of immobilized methods on the co-immobilization of tannase and β-glucosidase were investigated in this work. The results showed that the best co-immobilization could be obtained by selecting sodium alginate as the immobilized carrier and using the crosslinking-embedding-crosslinking method. The conditions of co-immobilization were also optimized, under which the activity recoveries of tannase and β- glucosidase could reach 67.3 % and 46.0%, respectively.

关 键 词：单宁酶 Β-葡萄糖苷酶 海藻酸钠 壳聚糖 共固定化 

分 类 号：Q814.2[生物学—生物工程]