表没食子儿茶素没食子酸酯诱导细胞凋亡蛋白酶途径依赖的人胃癌细胞株MKN45凋亡  

Epigallocatecnin-3-gallate Induces Caspase-3-dependent Apoptosis in Human Gastric Cancer Cell Line MKN45

作　　者：冉志华[1] 许琦[1] 陈翔[1] 萧树东[1] 

机构地区：[1]上海交通大学医学院附属仁济医院消化内科上海市消化疾病研究所,200001

出　　处：《胃肠病学》2006年第2期82-86,共5页Chinese Journal of Gastroenterology

摘　　要：背景:表没食子儿茶素没食子酸酯(EGCG)可诱导人胃癌细胞株MKN45凋亡,但其凋亡信号的传导途径尚不清楚。目的:研究EGCG诱导人胃癌细胞株MKN45凋亡的作用是否通过细胞凋亡蛋白酶(caspase-3)依赖途径,为其临床应用提供进一步的理论依据。方法:采用四甲基偶氮唑蓝(MTT)比色法检测EGCG和caspase-3抑制剂z-DEVD-fmk 作用后MKN45细胞的存活率;采用Annexin V-FITC+PI双染色法检测EGCG和caspase-3抑制剂作用后MKN45细胞的凋亡率;采用酶联免疫吸附测定(ELISA)检测EGCG和caspase-3抑制剂作用后MKN45细胞内caspase-3活性的改变。结果:EGCG可诱导MKN45细胞凋亡,且细胞内caspase-3活性显著升高。而caspase-3抑制剂干预后,EGCG抑制 MKN45细胞生长的作用明显减弱,细胞凋亡率下降,caspase-3活性显著下降。结论:EGCG可诱导MKN45细胞凋亡, 该作用可被caspase-3抑制剂显著抑制,提示EGCG诱导MKN45细胞凋亡的作用是通过caspase-3依赖途径的。Background： Epigallocatechin-3-gallate （EGCG） has been demonstrated to induce apoptosis of human gastric cancer cell hne MKN45, however, the apoptotic pathway triggered by EGCG is not yet clear. Aims： To investigate whether EGCG- induced apoptosis of gastric cancer cell line MKN45 was on caspase-3-dcpendent pathway, and to provide further evidence for its clinical application. Methods： The survival rate of MKN45 cells after treatment with EGCG and caspase-3 inhibitor z-DEVD-fmk was detected by methyl thiazolyl tetrazolium （MTT） assay. The apoptotic rate of MKN45 cells after EGCG and caspase-3 inhibiter treatment was evaluated by Annexin V-FITC＋PI dual staining. The influence of EGCG and caspase-3 inhibitor on the activity of caspase-3 in MKN45 cells was determined by enzyme-linked immunosorbent assay （ELISA）. Results： EGCG could induce apoptosis of MKN45 cells, and the activity of caspase-3 in MKN45 cells was apparently increased. However, after treatment with caspase-3 inhibitor, the inhibitory effect of EGCG on MKN45 ceils was significantly weakened, the apoptotic rate of the cells and the activity of caspase-3 also decreased dramatically. Conclusions： EGCG can induce apoptosis of MKN45 cells, and caspase-3 inhibitor can significantly inhibit the apoptosis induced by EGCG in MKN45 cells, The apoptotic pathway triggered by EGCG in MKN45 cells was caspase-3-dependent.

关 键 词：表没食子儿茶素没食子酸酯 胃肿瘤 细胞系 细胞凋亡蛋白酶 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]