胰腺实性-假乳头状肿瘤βcatenin及相关基因表达  

Expression of β-catenin and related genes in solid pseudopapillary neoplasms of pancreas

作　　者：吕炳建[1] 朱涛[2] 来茂德[1] 金梅[2] 许敬尧[2] 

机构地区：[1]浙江大学医学院病理学与病理生理学系,杭州310031 [2]浙江大学附属邵逸夫医院病理科,杭州310016

出　　处：《临床与实验病理学杂志》2006年第1期57-60,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的研究βcatenin及相关基因在胰腺实性-假乳头状肿瘤(solidpseudopapillaryneoplasmofpancreas,SPN)中的表达,探讨它们在SPN发生中的作用。方法回顾性复习7例SPN的临床和病理资料,并检测βcatenin、cyclinD1、p53等基因的蛋白表达。结果7例SPN都有βcatenin的异常染色(>50%的肿瘤细胞有细胞质和细胞核着色)、上皮钙黏蛋白细胞膜表达降低和p53低表达(<10%),p53表达在1例伴肝转移的SPC表达最高(>5%)。3例SPNcyclinD1过度表达(>10%),4例低表达(<5%);5例SPNMIB1+细胞<1/HPF,2例SPNER或PR+。结论βcatenin及相关基因的表达异常在SPN发生中起重要作用。Purpose To detect the gene expression alterations of β-catenin and related gene expressions in solid pseudopapillary neoplasm of pancreas （SPN） and to discuss their roles in the initiation and progression of SPN. Methods Seven SPNs, including two solid pseudopapillary carcinomas （SPC）, were studied via clinicopathological data review and immunohistochemical staining against a panel of antibodies including β-catenin, E-cadherin, cyclin D1, MIB1 and p53, Results All SPNs displayed β-catenin abnormal cytoplasmic and nuclear staining （ 〉 50% ） and low-expression of membranous E-cadherin staining and p53 （ 〈 10% ）. However, highest p53 expression （ 〉5% ） was identified in 1 SPC with liver metastasis, cyclin D1 was overexpressed in 3 SPNs （ 〉 10% ） and low in the remaining 4 cases （ 〈 5% ）. Five out of 7 SPNs was of low MIB-1 positive cells （ 〈 1/HPF）. However, only 2 cases were ER or PR positive. Conclusions Abnormal expressions of β-catenin and other downstream target genes, might play a pivotal role in the initiation and progression of SPN.

关 键 词：胰腺肿瘤 Β-CATENIN CYCLIN D1 免疫组织化学 

分 类 号：R735.9[医药卫生—肿瘤]