β_1整合素反义寡核苷酸对人胰腺癌细胞BXPC-3生长和化疗敏感性的影响  

The Effect of Integrin-β_1 Antisense Oligodexynucleotides on Cell Growth and Chemosensitivity of Pancreatic Cancinoma Cell BXPC-3

作　　者：黄涛[1] 常青[1] 高军[1] 冯延平[1] 夏维[1] 秦仁义[1] 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科,武汉市430030

出　　处：《中国肿瘤临床》2006年第4期186-189,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:30471963)

摘　　要：目的:观察β1整合素反义寡核苷酸(ASODNs)对人胰腺癌细胞BXPC-3β1整合素mRNA表达及细胞生长和对化疗药物敏感性的影响。方法:采用脂质体将不同浓度β1整合素ASODNs转染到人胰腺癌细胞BXPC-3中,逆转录-聚合酶链反应(RT-PCR)检测细胞β1整合素mRNA表达水平,MTT法检测细胞相对存活率。转染β1整合素ASODNs24h后,给予梯度浓度5-氟尿嘧啶(5-FU)、阿霉素和健择,观察量效关系,计算IC50。结果:与对照组相比,32mg/L～128mg/LASODNs转染后可抑制胰腺癌细胞的生长,且呈量效依赖关系,64mg/L和128mg/L抑制作用最明显,其对应的β1整合素mRNA表达水平显著降低。转染β1整合素ASODNs能增加胰腺癌细胞对化疗药物的敏感性,ASODNs+化疗组对各化疗药物的IC50均有显著下降。结论:靶向β1整合素的反义寡核苷酸可有效阻断其在人胰腺癌细胞中的表达,抑制胰腺癌细胞生长,增强对化疗药物敏感性。Objective： To investigate the effects of integrin-β1 antisense oligodexynucleotides （ASODNs） on cell-growth and chemosensitivity of pancreatic cancinoma cell line BXPC-3. Methods： Integrin-β1 antisense oligodexynucleotides was transfected into BXPC-3 with liposome. The expression of integrin-β1 mRNA was determined by RT-PCR. The viability rate was assessed by MTr method. Different concentrations of 5-FU, adriamycin （ADM）, Gemcitabine were given 24 h after cells transfected with integrin-β1 ASODNs. The dose-effects responses were observed and ICso s were calculated. Resuits： Transfecting BXPC-3 cells with 32mg/L-128mg/L integrin-β1 ASODNs inhibited growth significantly and reduced expression of integrin-β1 mRNA. Integrin-β1 ASODNs increased the ehemosensitivi- ty of BXPC-3 cells to 5-FU, ADM, Gemeitabine. The ICso s of different chemotherapy groups were sig- nificantly lower than those in controls. Conclusions： Integrin-β1 ASODNs can effectively decrease the expression of integrin-β1 gene, inhibits cell growth and enhances the ehemosensitivity in pancreatic eaneinoma cell line BXPC-3.

关 键 词：反义寡核苷酸 Β1整合素 胰腺肿瘤 化疗敏感性 

分 类 号：R735.9[医药卫生—肿瘤]