Genistein对腺样囊性癌细胞SACC-83中细胞周期蛋白表达的影响  

Effects of genistein on the expressions of cell cycle proteins in salivary adenoid cystic carcinoma cell line SACC-83

作　　者：马杰[1] 王洁[2] 钟鸣[2] 王兆元[2] 

机构地区：[1]中国医科大学第二临床学院口腔科,辽宁沈阳110004 [2]中国医科大学口腔医学院病理科,辽宁沈阳110002

出　　处：《上海口腔医学》2006年第1期69-72,共4页Shanghai Journal of Stomatology

基　　金：辽宁省教育厅科学研究计划资助(05L533)

摘　　要：目的:研究酪氨酸蛋白激酶抑制剂Genistein诱导人唾液腺腺样囊性癌细胞株SACC-83细胞周期阻滞的分子机制。方法:用Genistein处理体外培养的SACC-83细胞,采用Western印迹技术检测CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达,并利用电泳凝胶成像分析软件,对其结果进行量化分析,采用SPSS11.5统计软件对结果进行方差分析。结果:随着Genistein作用时间的延长和浓度的增加,CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达明显减少。SACC-83细胞经220μmol/LGenistein作用3d,其CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达量分别是对照组的58%、64%和46%、43%,差异非常显著(P<0.01)。结论:Genistein诱导SACC-83细胞周期阻滞于G2/M期,与其下调CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达有关。PURPOSE： To investigate the molecular mechanism of cell cycle arrest induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83. METHODS： SACC-83 cells cultured in vitro were treated with genistein, the expressions of CyclinB1,Cdkl,CyclinD1 and Cdk4 proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.5 software. RESULTS： With the increase of concentration of genistein and the elongation of time, the expression of CyelinB1,Cdkl,CyelinD1 and Cdk4 proteins was significantly decreased. Treated with 220μmol/L of genistein for 3 days, the expression level of CyclinB1, Cdkl, CyclinD1 and Cdk4 proteins was 58%,64%,46% and 43% of the control group, respectively （P〈0.01）. CONCLUSION： The cell cycle G2/M arrest induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the downregulations of CyclinB1, Cdkl, CyclinD1 and Cdk4 protein expressions. Supported by Scientific Research Plan of Education Bureau of Liaoning Province （05L533）.

关 键 词：酪氨酸蛋白激酶 GENISTEIN 唾液腺 腺样囊性癌 细胞周期 

分 类 号：R739.8[医药卫生—肿瘤]