蛋白激酶C抑制剂对小鼠腹腔巨噬细胞清道夫受体功能的影响  

Effect of Protein Kinase C Inhibitor on Scavenger Receptor in Mouse Peritoneal Macrophage

作　　者：周春蕾[1] 陈琪[2] 金艳[2] 陈秀英[2] 王南[2] 

机构地区：[1]中国人民解放军第454医院心内科,江苏省南京市210002 [2]南京医科大学动脉粥样硬化研究中心,江苏省南京市210029

出　　处：《中国动脉硬化杂志》2005年第5期533-536,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助项目(30370576)

摘　　要：目的观察细胞内蛋白磷酸化水平对清道夫受体功能的影响。方法用蛋白激酶C抑制剂星形孢菌素处理小鼠腹腔巨噬细胞,利用蛋白质印迹试验和放射自显影方法观察药物对细胞表面受体表达的影响,并分别测定对照组和处理组细胞对碘标记的氧化型低密度脂蛋白的结合、降解以及细胞内脂质蓄积的程度。结果0.4μmol/L蛋白激酶C抑制剂星形孢菌素可以促进细胞结合碘标记的氧化型低密度脂蛋白,增加细胞表面受体的表达,但抑制细胞降解碘标记的氧化型低密度脂蛋白,同时抑制细胞内胆固醇酯的蓄积。结论以上表明清道夫受体功能与细胞内蛋白质磷酸化水平密切相关。Aim To study effects of cell protein phosphorylation on scavenger receptor. Methods Mouse peritoneal maerophages （MPM） were treated with protein kinase C （PCK） inhibitor staurospofine, and then the cell were incubated with ^125I- （oxidized low density lipoprotein （ox-LDL） or ox-LDL. The effects of the drug on expression of cell surface receptor were observed by means of autoradiography. Moreover, the cellular binding of ^125I- ox-LDL, its degradation and the accumulation of lipids within cell were measured separately. Results Staurospofine （0.4 μmol/L} could enhance MPM to bind lipids and stimulate scavenger receptor exression, and could reduce degradation of lipids by MPM and the accumulation of cholestrol within the cell. Condusion The function of scavenger receptors may be correlated with cell protein phorsphorylation.

关 键 词：病理学与病理生理学 蛋白激酶C抑制剂对清道夫受体功能的影响 蛋白质印迹试验 清道夫受体 巨噬细胞 蛋白激酶C抑制剂 氧化型低密度脂蛋白 

分 类 号：R363[医药卫生—病理学]