草鱼生长激素基因在毕赤酵母中的表达  

Expression of Grass Carp Growth Hormone in Pichia pastoris

作　　者：孟亚鹏[1] 徐敏[1] 代焕梅[1] 丁秀琼[1] 张海英[1] 殷雪[1] 刘世贵[1] 龙章富[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川动物》2006年第1期68-71,共4页Sichuan Journal of Zoology

基　　金：教育部重点项目(国教00104);四川省"十五"重点项目(03NC002-002);四川省应用基础研究项目(02NY029-079)

摘　　要：将草鱼生长激素基因(cGH)的cDNA亚克隆到酵母表达载体pPIC9K中,经电击转化导入毕赤巴斯德酵母GS115菌株,获得转化子。菌落PCR技术筛选证实cGH已经整合到了酵母染色体上。对重组酵母进行诱导表达,SDS-PAGE和Western印迹分析,结果表明cGH在毕赤巴斯德酵母GS115菌株中获得了高效表达。The ORF of grass carp growth hormone （cGH） was isolated and cloned from pGEX4T-1-eGH plasmid and inserted into pPIC9K, expression vector. The Pichia postoris yeasts strain GS115 were transformed by electrophoration with pPIC9K-cGH and were screened on medium with G418. Colony PCR results showed that CGH gene had been integrated into the yeast chromosomes. By analyses of the SDS-PAGE and Western blotting, it was showed that cGH protein was expressed in Pichia pastoris GS115.

关 键 词：草鱼 生长激素基因 pPIC9K表达载体 毕赤酵母 高效表达 

分 类 号：Q786[生物学—分子生物学] S963.73[农业科学—水产养殖]