精子蛋白Sp32/OY-TES-1基因mRNA表达的探讨  

THE STUDY OF mRNA EXPRESSION IN SPERM PROTEIN Sp32/OY-TES-1 GENE

作　　者：范蓉[1] 肖绍文[2] 何少健[1] 张小静[1] 罗昱[2] 罗国容[1] 谢小薰[1] 

机构地区：[1]广西医科大学基础医学院组织学胚胎学教研室,南宁530021 [2]广西医科大学第一临床医院神经外科,南宁530021

出　　处：《解剖学报》2006年第1期102-105,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30360026);广西科学基金资助项目(0229032)

摘　　要：目的了解精子蛋白Sp32/OY-TES-1基因mRNA的表达特点,研制癌-睾丸抗原(CTA)的肿瘤疫苗。方法采用逆转录-聚合酶链反应(RT-PCR)技术,检测95例正常人组织及125例人肿瘤组织中Sp32/OY-TES-1mRNA的表达,随机选取RT-PCR阳性产物12份进行DNA测序;对所得数据进行统计学分析。结果Sp32/OY-TES-1在正常和肿瘤组织中的表达频率分别为68.4%(65/95)和44.00%(55/125),两者比较有统计学意义(P<0.05)。在24种不同器官的正常组织中,21种表达Sp32/OY-TES-1基因mRNA(87.50%)。DNA测序结果证实,RT-PCR产物为Sp32/OY-TES-1基因。结论Sp32/OY-TES-1基因的mRNA在正常组织中广泛表达,它是否可归为睾丸和肿瘤限制性表达的基因尚需进一步研究。Objective To investigate the mRNA expressive profile of Sp32/OY-TES-1 gene. Methods Ninety-five spcimens of human normal tissues and 125 specimens of human tumor tissues were analyzed for the expression of OY-TES-1 by reverse transcription polymerase chain reaction （RT-PCR）. Twelve RT-PCR positive products were randomly selected and sequenced. The data obtained were staticly analized. Results The expressive frequencies of Sp32/OY-TES-1 in normal and tumor tissues were 68.42 % （65/95） and 44.00 % （55/125 ）, respectively, which were of significant difference （ P 〈 0.05 ）. On 24 specimens of normal tissues from different organs, 21 were positive in Sp32/OY-TES-1 （87.50 % ）. DNA sequencing results further confirmed that RT-PCR products were truly Sp32/OY-TES-1 eDNA. Conclusion With ubiquitous expression in human normal tissues, whether Sp32/OY-TES-1 is a gene expressed strictly in testis and tumor tissues or not needs to be further determined.

关 键 词：癌-睾丸抗原 基因表达 精子蛋白 逆转录聚合酶链反应 人 

分 类 号：R730.3[医药卫生—肿瘤]