发酵秸杆生产耐高温纤维素酶工艺条件的研究  

Study on the process conditions of high-temperature-tolerant cellulase produced by fermentation of straw stalk

作　　者：夏服宝[1] 孙宪迅[2] 刘德超[1] 邱雁临[1] 关迅[3] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]江汉大学医学与生命科学学院,湖北武汉430056 [3]湖北生物科技职业学院,湖北武汉430070

出　　处：《中国酿造》2006年第3期18-20,共3页China Brewing

摘　　要：对绿色木霉110发酵秸秆生产纤维素酶工艺条件进行了研究,通过正交试验和扩大试验,确定了绿色木霉固体发酵生产纤维素酶的最佳工艺条件为干料:水=1:1,原料配比(稻草:麸皮)为6:4,容器装料量7%,接种量3%,培养温度38℃,发酵周期72h,试验因素的影响顺序为水分>原料配比>装料量>接种量。在最佳工艺条件下,产酶酶活超过25300U/g。The fermentation conditions of straw stalk to produce cellulase with the strain Trichoderma viride 110 were studied. The optimal fermentation conditions were： water-dried material ratio was 1：1, raw materials ratio （straw ： bran） was 6：4, added material was 7%, inoculums was 3%, temperature was 38℃, and fermentation cycle was 72 h. Water was the decisive factor, which influences the technical conditions. The sequence of influence was： water 〉 ratio of raw materials 〉 added material 〉 inoculums. Under the optimum technical conditions, the activity of enzyme reached 25300 U/g.

关 键 词：发酵秸秆 纤维素酶 正交试验 工艺条件 

分 类 号：Q814[生物学—生物工程]