肿瘤坏死因子相关凋亡诱导配体受体DR4在大肠癌的表达及意义  

Expression and significance of TNF-related apoptosis-inducing ligand receptor DR4 in large intestine carcinoma

作　　者：闫庆辉[1] 张国建[1] 任鹏涛[1] 赵晶[1] 王凤安[1] 

机构地区：[1]河北医科大学第二医院胃肠外科,河北省石家庄市050000

出　　处：《世界华人消化杂志》2006年第3期332-335,共4页World Chinese Journal of Digestology

基　　金：河北省卫生厅科学基金资助项目;No.2004014~~

摘　　要：目的:探讨肿瘤坏死因子相关凋亡诱导配体受体DR4在大肠癌组织中的表达情况及其与临床病理的关系．方法:采用免疫组化SP法和流式细胞分析方法对41例大肠癌组织和9例正常组织DR4的表达情况进行检测并比较．结果:DR4蛋白免疫组化染色定位于细胞膜和细胞浆,癌组织中阳性表达率为65．85％,明显高于正常组织的22．20％(P<0．05)．流式细胞分析该蛋白表达量,癌组织平均荧光指数为1．17±0．13,亦明显高于正常组织的1．00± 0．13(P<0．05)．不同分化程度的癌组织中DR4 蛋白表达无统计学差异,临床有淋巴结转移的癌组织与无淋巴结转移的癌组织DR4表达无统计学差异．结论:大肠癌组织中DR4表达上调,与组织分化程度无关．AIM： To explore the expression of TNF-related apoptosis-inducing ligand （TRAIL） receptor DR4 in large intestinal carcinoma and its correlation with clinical pathological characteristics. METHODS： SP immunohistochemical technique and flow cytometry were used to detect the expression of DR4 in 41 cases of large intestinal carcinoma and 9 cases of normal tissues. RESULTS： The positive cell membrane and cytoplasmic expression were observed in large intestinal carcinoma and normal large intestine mucosal tissues. Of large intestinal carcinoma, DR4 was positively expressed in 65.85%. However, the positive rate of DR4 in the normal tissues was 22.20%, which was significant lower than that in the cancer tissues （P 〈0.05）. The fluorescence index （FI） of DR4 protein in the normal mucosal tissues was 1.00±0.13, while that in the cancer tissues was 1.17±0.13. There was significant difference between them （P 〈0.05）. DR4 expression in the cancer tissues was not correlated with the degree of differentiation and lymph node metastasis. CONCLUSION： The expression of DR4 increases in large intestinal cancer tissue, but it is not correlated with differentiation.

关 键 词：大肠癌 DR4 免疫组化 流式细胞分析 

分 类 号：R735.34[医药卫生—肿瘤]