一种适合于溶葡球菌酶发酵生产和分离纯化的半合成培养基的建立  

A semi-synthetic medium suitable for fermentational production and purification of lysostaphin

作　　者：张培德[1] 夏晴[1] 陈石根[1] 

机构地区：[1]复旦大学生物化学系

出　　处：《复旦学报（自然科学版）》1996年第2期157-162,共6页Journal of Fudan University：Natural Science

摘　　要：利用含溶葡球菌酶基因的枯草芽孢杆菌（ＢＲ１５１）转化子进行半合成培养基的发酵试验，结果表明：该半合成培养基适合于枯草芽孢杆菌生长，色素极微．半合成培养基蛋白总量是ＦＤ培养基的１／２４．用半合成培养基摇瓶发酵１４ｈ后，产酶量在（３７０±２６）ｍｇ／Ｌ．表明该培养基将有利于溶葡球菌酶的分离纯化，是溶葡球菌酶发酵生产的一个理想培养基．A transformant of Bacillus subtilis BR151 with lysostaphin gene grown in semi-synthetic medium is discovered. The medium is good for both the growth of Bacillus subtilis and enzyme synthesis. The colour of the medium is light and its protein content is extremely low. Separation and purification of lysostaphin from the medium is therefore easy. After 14 h of culture, the strain BR151 produces(370 ± 26 ) mg/L of lysostaphin.

关 键 词：半合成培养基 枯草芽孢杆菌 溶葡球菌酶 发酵 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]