PCR-SSCP法检测非小细胞肺癌中FADD基因突变的研究  

PCR-SSCP Analysis of FADD Gene Mutation in Non-small Cell Lung Cancer

作　　者：杨琴[1] 陈红雷[2] 刘铭球[2] 方忠[3] 

机构地区：[1]三峡大学医学院病理学教研室,湖北宜昌443000 [2]武汉大学医学院病理教研室 [3]华中科技大学同济医学院附属同济医院外科

出　　处：《肿瘤防治研究》2006年第3期162-164,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(39870305)

摘　　要：目的检测Fas相关死亡结构域蛋白(Fasassociateddeathdomainprotein,FADD)基因在人非小细胞肺癌(nonsmallcelllungcancer,NSCLC)中的突变情况,以探讨该基因在肺癌发生发展中的作用及机制。方法采用聚合酶链反应-单链构象多态性分析(polymerasechainreactionandsinglestrandconformationpolymorphism,PCRSSCP)检测74例NSCLC原发灶癌组织及13例癌旁正常肺组织中FADD基因突变情况。结果74例NSCLC组织中检出5例发生FADD基因突变,FADD基因突变与癌的淋巴结转移呈显著正相关(rs=0.378,P=0.001),与其他临床病理特征无关。结论NSCLC中存在着FADD基因突变。FADD基因突变在NSCLC的发生发展中可能起着重要作用。Objective This report aimed at the mutation of FADD gene in human non-small cell lung cancer, and the evaluation of its possible effect and mechanism on the development of NSCLC. Methods PCR-SSCP was used to detect FADD gene mutation in 74 specimens from primary site of NSCLC tissues and 13 samples of normal adjacent lung tissues. Results Altogether there were 5 cases of FADD gene mutation, and the analysis revealed that its mutation was positively correlated to the status of lymph node metastasis （rs = 0. 378, P = 0. 001 ）, but no relationship was found between FADD mutation and other clinical features. Conclusion There is FADD gene mutation in NSCLC. FADD gene mutation probably plays a crucial role in NSCLC carcinogenesis.

关 键 词：肺肿瘤 突变 FADD基因 PCR-SSCP 

分 类 号：R734.2[医药卫生—肿瘤]