Genistein对耐药卵巢癌细胞SKOV-3增殖、凋亡和顺铂敏感性的影响  

Effects of Genistein on Proliferation, Apoptosis and Cisplatin Sensitivity of Drug-resistant Human Ovarian Carcinoma Cell SKOV-3

作　　者：袁鹏[1] 黄艳红[1] 辛晓燕[1] 宋晖[1] 田爽[1] 于月成[1] 王德堂[1] 

机构地区：[1]第四军医大学西京医院妇产科,西安710032

出　　处：《肿瘤防治研究》2006年第3期187-190,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30472226)

摘　　要：目的探讨Genistein对耐药卵巢癌细胞SKOV3增殖、凋亡和顺铂敏感性的影响。方法采用MTT法检测Genistein单用及合用顺铂对细胞增殖的影响,流式细胞仪分析Genistein及Genistein联合顺铂对细胞周期和凋亡的影响。结果Genistein对SKOV3细胞增殖表现出浓度依赖性的抑制作用,并显著提高了其对顺铂的敏感性(P<0.05);0.625～5μg/ml顺铂与2.5～10μg/mlGenistein合用基本表现为协同作用。5、10μg/mlGenistein均可将细胞阻滞于G2/M期并诱导一定程度的凋亡,10μg/mlGenistein还可进一步的干扰S期进程;当顺铂与Genistein合用时,两种药物在干扰SKOV3细胞周期和诱导凋亡方面表现为显著的协同效应。结论Genistein能够抑制耐药卵巢癌细胞SKOV3的增殖,并显著增强该细胞对顺铂的敏感性。Objective To study the effects of genistein on proliferation, apoptosis and cisplatin sensitivity of drug-resistant ovarian carcinoma cell skov-3. Methods MTT assay was used to analyze growth inhibitory effect of genistein alone and with cisplatin on SKOV-3 cell. Cell cycle arrest and apoptosis was measured by flow cytometry （FCM）. Results Genistein exerted inhibitory effect on SKOV-3 cell in a concen-tration-dependent way and significantly enhanced its sensitivity to cisplatin （P 〈0. 05）. Generally, cisplatin （0. 625 -5μg/ml） combined with genistein （2. 5-10μg/ml） resulted in synergistic effect. Both 5μg/ml and 10μg/ml genistein arrested SKOV-3 cell in the G2/M phase and induced apoptosis while 10μg/ml genistein caused additional disturbance on S phase process. Significant synergistic effect between genistein and cisplatin was found in inducing cell cycle arrest and apoptosis. Conclusion Genistein can inhibit the growth of drug-resistant human ovarian carcinoma cell SKOV-3 and enhance its sensitivity to cisplatin significantly.

关 键 词：GENISTEIN 顺铂 卵巢癌 耐药 

分 类 号：R730.53[医药卫生—肿瘤] R737.31[医药卫生—临床医学]