豫医无毛小鼠无毛基因部分内含子突变研究  

Mutations in Intron of YYHL Hairless Gene

作　　者：臧文巧[1] 马一君[2] 杨璇[1] 周晓丽[3] 李敏[1] 李倩如[1] 

机构地区：[1]郑州大学医学院微生物学与免疫学教研室,河南郑州450052 [2]河南中医学院病原与免疫学科,河南郑州450008 [3]郑州市第五人民医院,河南郑州450003

出　　处：《河南职工医学院学报》2006年第1期4-6,共3页Journal of Henan Medical College For Staff and Workers

基　　金：国家自然科学基金资助项目(No39970119)

摘　　要：目的研究豫医无毛小鼠突变的分子机制。方法对豫医无毛小鼠和昆明小鼠的基因组DNA进行PCR扩增,对二者扩增片段克隆后测序,对结果进行比较,以确定豫医无毛小鼠无毛基因的特异性程度。结果本实验共测出豫医无毛小鼠DNA序列1 746 bp和昆明小鼠DNA序列1 733 bp,两者不同之处有32处,均位于内含子,突变形式包括插入、缺失和点突变。结论无毛性状的产生可能与内含子中的插入、缺失和点突变有关。Objective To research the gene mutation of Yuyi hairless mice （for short： YYHL）. Methods The partial hairless gene of YYHL, KM mice, Mus musculus （house mouse） were cloned, sequenced andThe partial hairless gene of YYHL, KM mice, Mus musculus （house mouse） were cloned, sequenced and compared respectively. Results The DNA fragment sequenced of YYHL mice contains 1746 bp, KM mice 1733bp .There were 32 differences in intron, including insertion, deletion and point mutation. Conclusion The hairless character is probably associated with the gene mutation of intron .

关 键 词：豫医无毛小鼠 基因 突变 内含子 

分 类 号：R394.33[医药卫生—医学遗传学]