苦瓜种子发育过程中水溶性蛋白组分的研究  

Studies on the water-soluble protein composition in seeds of Momordica charantia L.at different developmental stages

作　　者：孙海燕[1] 童富淡[2] 胡家恕[3] 汪俏梅[1] 

机构地区：[1]浙江大学园艺系 [2]浙江大学动物科学学院,浙江杭州310029 [3]浙江大学生命科学学院,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2006年第2期134-138,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30000015);浙江省科技计划资助项目(2004C32014)

摘　　要：选取在果型和果色上具典型性的4个苦瓜品种为材料，对不同发育时期种子的可溶性蛋白提取物进行了SDS-PAGE和IEF分析．结果表明，所有品种苦瓜种子均含有丰富的可溶性蛋白（14.4～116.0kD），其中以分子量为27．6，47．4和43．4kD的蛋白含量较高，有3种蛋白的分子量在30kD左右，不同品种之间在小分子量蛋白的表达上有一定差异；所有品种种子均含有4种以上的碱性蛋白，但不同品种之间碱性蛋白的含量和积累规律存在一定的差异．SDS-PAGE and IEF analysis of water-soluble proteins were conducted on seeds of 4 typical Momordica charantia L varieties at different developmental stages, and the results indicated that the seeds contained abundant water-soluble proteins, the molecular weights of which were ranging between 14.4 and 116kD, among them, the 27.6, 47.4 and 43.4 kD proteins were present in relatively high content, and the molecular weights of three other proteins were roughly 30 kD. There existed some difference in the expression patterns of the small molecular weight proteins among different varieties. At least 4 alkaline proteins were common in all varieties tested, although they differed in amount and pattern of accumulation among different varieties.

关 键 词：苦瓜 种子 水溶性蛋白 SDS-PAGE IEF 

分 类 号：S482.8[农业科学—农药学]