硒蛋白抑制肿瘤的动物试验  

Antitumor activity of selenoprotein against mouse tumor

作　　者：朱功华 兰宁 徐朝斌 

机构地区：[1]三明市中西医结合医院,福建三明365001 [2]三明市第一人民医院,福建三明365000 [3]厦门市第二人民医院,福建厦门361000

出　　处：《基层医学论坛》2006年第3期195-196,共2页The Medical Forum

摘　　要：目的采用自富硒豌豆中提取的硒蛋白进行抑瘤试验。方法受试硒蛋白制成经酶解和非酶解两种形式,给接种了小鼠肉瘤S180和小鼠肝癌HAC的ICR纯系小鼠分组饲以不同剂量和两种形式的硒蛋白口服液。结果两种硒蛋白液对S180的生长均有一定的抑制作用,大剂量组的抑制率分别达52.24%和55.88%,与对照组有显著差异(P<0.001)。两种硒蛋白使HAC荷瘤鼠的存活期分别可延长37.30%和27.78%。与对照组相比有显著差异(P<0.001)。结论饲用酶解硒蛋白的HAC荷瘤鼠其存活期高于饲用非酶解硒蛋白而与5-Fu相当。Objective The pea selenoprotein, a plant protein, purified from the extracts of pea seeds, binding selenium at amino acid residus （containing Se 200ppm）, were demonstrated their antitumor activity. Methods We prepared a water-souble oral liquid and hydrolyzed oral liquid byprotein enzyme. The mouse sarcoma S180 and mouse hepatocareinoma HAC were inoculated into the nude mice. Two kinds of selenoprotein were given orally to the mice, 0.1～0.3 ml/10 g per daily for ten days. Results It showed both selenoprotein inhibit the growth of the mouse S180, particularly at maximal dosage （0.3 ml/10 g）. The inhibition rate to S180 were 55.88% and 52.24% in two kinds of selenoprotein respectively. There was a extremely difference between test groups and the control groups （P 〈 0.001）, For HAC mice, two kinds of selenoprotein＇also prolonged the survival time, prolonged survival rate 37.3% and 27.78% （P 〈 0.001）. It exerted an anti heptocareinoma effect equivalent to that of 5-Fu in the groups given the hydrolyzed selenoptein. Conclusion These data suggested that pea selenoprotein has a high antitumor activity and is a potential therapeutic agent.

关 键 词：硒蛋白 抑瘤试验 

分 类 号：R73-36[医药卫生—肿瘤]