地黄寡糖在2型糖尿病大鼠模型上的降血糖作用及机制  

Hypoglycemic effect of rehmannia glutinosa oligosaccharide in rats with type 2 diabetes

作　　者：曾艳[1] 贾正平[2] 张汝学[2] 李茂星[2] 王娟[2] 胡静[2] 

机构地区：[1]兰州大学生命科学学院,甘肃兰州730000 [2]兰州军区兰州总医院全军临床药理基地,甘肃兰州730050

出　　处：《中国药理学通报》2006年第4期411-415,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30472186);甘肃省自然科学基金资助项目(No3ZS051-A25-078)

摘　　要：目的研究地黄寡糖(ROS)在2型糖尿病(T2DM)动物模型和正常大鼠高血糖模型上对血糖的影响及机制。方法采用长期高脂肪饲料饲养加小剂量链脲佐菌素(STZ,30mg·kg-1,ip)诱导♀大鼠2型糖尿病动物模型;采用葡萄糖(2·5g·kg-1,ip)和肾上腺素(300μg·kg-1,ip)诱导正常♂大鼠高血糖模型。动物分为正常对照组、2型糖尿病模型组或高血糖模型组、ROS给药组及阳性对照二甲双胍组,以上各组均为灌胃给药。结果与2型糖尿病模型组比较,ROS高、低剂量组均可降低糖尿病大鼠的血糖值,以高剂量组最为明显;ROS对血浆总胆固醇(TC)、甘油三酯(TG)含量有降低趋势,对高密度脂蛋白胆固醇(HDL-C)含量有升高趋势;ROS可使肝脏葡萄糖-6-磷酸酶(G-6-Pase)活性降低,使肝糖原和胰岛素含量呈增加趋势;对正常大鼠肾上腺素性高血糖模型,ROS给药6d后,血糖峰值比高血糖模型组低。结论ROS灌胃给药对2型糖尿病大鼠的血糖有降低作用,其机制与降低肝葡萄糖-6-磷酸酶的活性有关,也可能与增加大鼠肝糖原合成和促进胰岛素分泌有关。ROS亦有降低大鼠肾上腺素性高血糖的作用。Aim To investigate the effect of rehmannia glutinosa oligosaccharide （ROS） on plasma glucose, lipid level, hepatic glycogen content, plasma insulin level and the influence on the hepatic key enzyme activity of glucose metabolism in type 2 diabetic rats. Methods According to the purpose of the experiment, type 2 diabetic modek was induced by both high lipid food and low dose of STZ in rats, which were divided into control group, type 2 diabetic model group, ROS treated group and metformin treated group. Fe- male Wistar rats were used in the experiment, with the number of 6-8 in each group. The positive control group was administered with metformin （200 mg · kg^-1 · d^-1, ig）. Results The plasma glucose level,plasma lipid level and the activities of glucose-6-phos- phatases in the high dose （200 mg · kg^-1 · d^-1） ROS treated group were decreased compared with thats of type 2 diabetic model group. On the other hand, the content of hepatic glycogen and plasma insulin level were higher than that of diabetic model group. Conclusion ROS has a significant hypoglycemic effect in type 2 diabetic rats. This effect may be related to the increase of hepatic glycogen level and insulin level and the decrease of activity of glucose-6-phosphatase.

关 键 词：地黄寡糖 链脲佐菌素 2型糖尿病 动物模型 降血糖 

分 类 号：R-332[医药卫生] R282.71