L-丝氨酸高产菌株的选育和摇瓶发酵条件优化  

Breeding of L-Serine-Producing Strain and Optimization of Fermentation Conditions

作　　者：魏东[1] 谭慧林[2] 杨海燕[2] 崔春生[1] 殷丽霞[2] 

机构地区：[1]新疆农科院微生物研究所,新疆乌鲁木齐830091 [2]新疆农业大学食品科学学院,新疆乌鲁木齐830052

出　　处：《氨基酸和生物资源》2006年第1期46-48,共3页Amino Acids & Biotic Resources

基　　金：国家十五攻关2004BA713B07-04

摘　　要：采用B revibacterium flavmC-11A为出发菌株,经紫外线照射和亚硝基胍诱变处理,选育出一株L-丝氨酸高产菌株C32为目的突变株,使摇瓶产酸率由12.1 g.L-1增加到16.4 g.L-1,然后对其进行摇瓶发酵条件优化,使菌株C32的L-丝氨酸产率提高到30.1 g.L-1。The strain Brevibacterium flavmC - 11A was mutated by ultraviolet radiation and NTG. As a result, a strain producing L - serine named C32 was obtained. The production of serine by flask - shaking fermentation increased from 12. 1g · L^-1 to 16.4g · L^-1 . Under optimum conditions, the production of serine by flask - shaking fermentation was up to 30. 1g · L^-1.

关 键 词：丝氨酸 选育 诱变 发酵 

分 类 号：TQ922[轻工技术与工程—发酵工程]