两步发酵法生产1，3-丙二醇的研究  

Investigation of Two Successive Bioprocesses for the Microbial Production of 1,3-propanediol

作　　者：刘海军[1] 徐友海[1] 张代佳[1] 修志龙[1] 

机构地区：[1]大连理工大学环境与生命学院生物科学与工程系

出　　处：《食品与发酵工业》2006年第2期4-7,共4页Food and Fermentation Industries

基　　金：科技部"十五"科技攻关计划项目(2001BA708301-04);"973"计划项目(2003CB716000)

摘　　要：通过几种不同原料制备的甘油对克雷伯杆菌厌氧发酵生产1,3-丙二醇的影响的比较实验,验证了两步发酵法生产1,3-丙二醇工艺路线的可行性。在甘油初始浓度相同(70g/L)的条件下,以葡萄糖为原料进行发酵得到的甘油转化为1,3-丙二醇的效果较好,发酵15 h,甘油转化为1,3-丙二醇的摩尔转化率为45．1%；皂化甘油生产1,3-丙二醇的发酵时间为18h,摩尔转化率为44．2%；而以玉米淀粉水解液发酵的甘油转化为1,3-丙二醇需要31 h,摩尔转化率仅为26．5%。An investigation of microbial production of 1,3-propanediol in a two-stage fermentation by using glucose or cornstarch as raw material was carried out in this work. In comparison with the anaerobic batch fermentation of 1,3-propanediol from saponified glycerol, the bioprocess of two successive microbial fermentations for the production of 1,3-propanediol was feasible. The yield of 1,3-propanedio was higher than that of 1,3-propanediol directly from saponified glycero with 15 h in the two-stage fermentation in a 18h batch fermentation. The molar yields of 1,3-propanediol to glycerol were 47.3 % and 44.2 %, respectively, at the same initial glycerol concentration of 70 g/L. The final concentration of 1,3 propanediol in a two-stage fermentation from glucose was 24.8 g/ L. This is almost same as that from saponified glycerol in single batch fermentation （24.2 g/L）. However, the conversion rate of glycerol produced from the first stage using cornstarch to 1,3-propanediol from the second stage was only 26.5 % （mol/mol） in 31h and the final concentration of 1,3-propanediol was 13.4 g/L. The productivity of 0.43 g/（L· h） of the second stage was much lower than that of a two-stage-fermentation from glucose （1.65g/ L） or of single fermentation from saponified glycerol, which is 1.34g/L.

关 键 词：1 3-丙二醇 甘油 葡萄糖 玉米淀粉 微生物发酵 

分 类 号：TQ223.162[化学工程—有机化工]