手性氨基酸的毛细管电泳拆分研究  

Chiral Separation of Amino Acids by Capillary Electrophoresis

作　　者：程燕[1] 白敏[1] 石运伟[1] 明永飞[1] 尤进茂[1] 

机构地区：[1]曲阜师范大学化学科学学院,山东曲阜273165

出　　处：《分析测试学报》2006年第2期52-55,59,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20075016)

摘　　要：以咔唑-9-乙基氯甲酸酯(CEOC)作柱前荧光衍生试剂,在毛细管区带电泳和胶束电动色谱条件下,以不同配比的β-环糊精(-βCD)和脱氧胆酸钠(SDC)作手性选择剂,在两种不同分离模式下,对氨基酸衍生物进行了手性分离,同时对分离过程中的相关参数进行了优化,分别实现了8种和10种氨基酸的快速手性分离。Simple and rapid methods for the chiral separation of amino acid enantiomers using 9-（2- carbazole） ethyl chloroformate （ EEOC ） as derivation reagent by capillary zone electrophoresis （CZE） and micellar electrokinetic chromatography（MEKC） with diode array detector at 235 nm have been developed. Based on cooperative effect of binary chiral selectors,β-eyclodextrin（β-CD） and sodium deoxycholate（SDC） were investigated as chiral selectors, and the optimum molar ratio of β-CD to SDC for the chiral separation of each of amino acid enantiomers was obtained. Under CZE and MEKC separation modes, eight and ten pairs of amino acid enantiomers were separated with a good baseline resolution respectively. At the same time, the effect of some important parameters on the chiral separation was also investigated.

关 键 词：手性分离 毛细管电泳 氨基酸 Β-环糊精 脱氧胆酸钠 

分 类 号：O657.8[理学—分析化学] Q517[理学—化学]