使用偏爱密码子大幅度提高苯丙氨酸脱氨酶在食品级乳酸乳球菌中的表达  

High-level Expression of Phenylalanine Ammonia-lyase in Lactococcus Lactis Via Synthesized Sequence Based on Bias Codons

作　　者：陈星[1] 高斌[1] 贾兴元[1] 苏畅[1] 吕月平[1] 王战勇[1] 范新萍[1] 肖白[1] 刘敬忠[1] 

机构地区：[1]首都医科大学附属朝阳医院基础医学研究中心

出　　处：《生物工程学报》2006年第2期187-190,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助项目(No.30271372)。~~

摘　　要：为获得苯丙氨酸脱氨酶(PAL)在食品级乳酸乳球菌中的高效表达,将欧芹palcDNA(palnat)及根据乳酸乳球菌偏爱密码子设计人工合成的pal基因(palart)重组并转化到两种乳酸乳球菌NICE诱导表达系统中,测定基因工程菌表达PAL酶的量及活性,对比分析密码子偏爱性对乳酸乳球菌表达外源蛋白的影响。结果表明在两种乳酸乳球菌NICE表达系统中,使用偏爱密码子均可显著提高PAL酶的表达效率,使NZ9000/pNZ8048表达系统表达量提高22.23倍,NZ3900/pNZ8149系统提高35.90倍。此研究获得了安全高效表达PAL,可用于治疗苯丙酮尿症的基因工程菌。To construct a safer and more efficient gene engineering Lactococcus Lactis for expressing phenylalaine ammonia lyase （PAL） which will be benefit for PKU therapy, pal cDNA of Parsly and synthesized sequence based on Lactococcus Lactis bias codons were recombined into two Lactococcus Lactis NICE systems. The activities of the expressed PAL were detected, and the effect of Lactococcus Lactis bias codons on the expression of exterior protein was analyzed. The results showed that the expression level of PAL was increased by using Lactococcus Lactis bias codons in both Lactococcus Lactis NICE systems. Through which several safer andmore efficient strains of the gene engineering Lactococcus Lactis were obtained.

关 键 词：苯丙氨酸脱氨酶 乳酸乳球菌 NICE表达系统 密码子 

分 类 号：Q78[生物学—分子生物学]