茶多酚及没食子酸表没食子酸儿茶素对实验性酒精性肝病大鼠肝脏的保护作用及机制研究  

Study of protective role and its mechanism of tea polyphenols and EGCG in rats with alcohol-induced liver disease

作　　者：周晓蓉[1] 龚作炯[1] 袁光金[1] 孙小梅[1] 

机构地区：[1]武汉大学人民医院感染科,湖北武汉430060

出　　处：《中国现代医学杂志》2006年第6期840-843,共4页China Journal of Modern Medicine

摘　　要：目的研究茶多酚及EGCG对实验性酒精性肝病大鼠肝脏的保护作用及其作用机制。方法将48只大鼠随机分为正常对照组、模型组、茶多酚治疗Ⅰ组、茶多酚治疗Ⅱ组、EGCG治疗Ⅰ组、EGCG治疗Ⅱ组。以酒精+0.5mL鱼油灌胃制作酒精性肝病模型。茶多酚治疗Ⅰ、Ⅱ组另外分别给予200mg/kg、100mg/kg茶多酚灌胃治疗,EGCGⅠ、Ⅱ治疗组则给予100mg/kg、50mg/kgEGCG灌胃治疗。4周后处死大鼠,观察各组大鼠肝脏病理变化,并测定肝功能。ELISA检测血清TNF-α、IL-1、IL-18水平。结果模型组大鼠血清ALT水平与正常对照组相比,显著升高,并且肝组织表现肝细胞中度脂肪变性,点状坏死,炎性细胞浸润。茶多酚及EGCG治疗组显著降低血清ALT水平,与正常组相接近,肝组织仍可见肝细胞脂肪变性,但未见坏死及炎性细胞浸润。模型组血清TNF-α、IL-1及IL-18水平与正常组相比显著升高,但给予茶多酚及EGCG治疗后,其水平均明显下降。结论茶多酚及EGCG可减轻酒精性肝损伤的炎症与坏死,其机制可能与降低炎症细胞因子TNF-α、IL-1和IL-18等有关。[Objective] To study the effects of tea polyphenols and EGCG on alcohol-induced liver disease and to explore its mechanisms. [Methods] Forty eight Wister rats were randomly divided into six groups： normal group, model group, two tea polyphenols treatment groups, and two EGCG treatment groups. Alcoholic liver injury was induced by garage of alcohol plus 0.5 mL fish oil for 4 weeks. Tea polyphenols treatment group rats were administered with 200 mg/kg, 100 mg/kg tea polyphenols respectively; EGCG treatment groups with 100 mg/kg, 50 mg/kg EGCG. Liver injuries were assessed by histopathologic examination and serum ALT levels. TNF-α, IL-1 and IL-18 serum levels were assayed by ELISA. [Results] Model group rats had marked increase in ALT levels compared with norreal group, and their liver tissue showed hepatoeytes fatty degeneration , focal necrosis and inflanmntory cells infiltration. However, tea polyphenols and EGCG treatments resulted in decreased ALT levels and improved pathological changes. Serum TNF-α, IL-1 and IL-18 levels were elevated significantly in model group compared with normal group, but markedly suppressed by tea polyphenols and EGCG treatments. [Conclusions] Tea polyphenols and EGCG prevent inflammation and neerosis in alcohol-induced liver disease, and its mechanism may involve the inhibition of inflammatory cytokines, such asTNF-α, IL-1 and IL-18.

关 键 词：酒精性肝病 茶多酚 EGCG TNF-α IL-1 IL-18 

分 类 号：R575[医药卫生—消化系统]