中华鲟硫酸软骨素提取工艺研究  

Study on the extraction technology for the chondroitin tultate from Aclpenser Sinenais Grdy

作　　者：舒坤贤[1] 张继承[1] 袁帅[1] 谢永芳[1] 粱亦龙[1] 

机构地区：[1]重庆邮电大学生物信息学院,重庆400065

出　　处：《食品工业科技》2006年第3期151-152,155,共3页Science and Technology of Food Industry

基　　金：重庆邮电大学年青科技基金资助(A2005-22)

摘　　要：以中华鲟软骨为原料,采用稀碱与酶解相结合的方法提取硫酸软骨素。以蛋白酶、脂肪酶分解去除杂蛋白及脂肪,经酒精沉淀并干燥后得到产品。实验得到的最佳提取工艺为:料液比1∶1.5,碱浓度5%,碱提温度50℃,酸性、中性和碱性蛋白酶的用量分别为1.4%、1.4%、0.7%,脂肪酶的用量1%,在酶各自最适条件下作用时间各为1～1.5h。A method of dilute alkali-enzyme hydrolysis to extract chondroitin sulfate from the Aclpenser Sinensis Grdy cartilage was studied. Chondroitin sulfate was precipitated by ethanol after removing the protein and fat with protease and lipase, and the product was obtained after drying. The best extraction technology is that the weight proportion is 1：1.5 （material：liquid）, the alkali concentration is 5 %, the temperature is 50℃, the dosage of acid, neutral and alkali protease is 1.4%, 1.4%, 0.7%, respectively, lipase is 1%, and the reaction time is 1~1.5h under their optimum conditions.

关 键 词：中华鲟 硫酸软骨素 提取工艺 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程]