大肠杆菌SerB基因的克隆及其在黄色短杆菌中的表达  

Cloning of Phosphoserine Aminotransferase (SerB) Gene of Escherichia coli and Expression in Brevibacterium flavum

作　　者：殷丽霞[1] 崔春生[2] 简子健[1] 谭慧林[2] 包慧芳[2] 

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]新疆农科院微生物所,新疆乌鲁木齐830000

出　　处：《新疆农业科学》2006年第2期96-99,共4页Xinjiang Agricultural Sciences

基　　金：国家"十五"重点攻关项目(2004BA713B07-04)

摘　　要：磷酸丝氨酸转氨酶(Phosphoserine aminotransferase,SerB)是L-丝氨酸生物合成中的关键酶,应用PCR技术从大肠杆菌JM109(E.ColiJM109)中扩增出磷酸丝氨酸转氨酶基因,将其与表达载体pEC 7连接。通过电转化方法,将重组质粒转入黄色短杆菌(Brevibacterium flavumC-11,BfC-11)中,经酶活检测和摇瓶发酵培养,含有重组表达质粒的BfC-11B的磷酸丝氨酸转氨酶的活力和L-丝氨酸产率均比原宿主菌BfC-11有所提高,为构建L-丝氨酸高产基因工程菌的研究奠定了基础。Phosphoserine aminotransferdse （SerB） is a key enzyme in biosynthesis of L- serine. The SerB gene is obtained from E. coli JM109 by PCR. This gene was inserted into pEC7 and the recombinant plasmids with SerB gene were transformed into Brevibacterim flavum C- 11（BfC- 11）by the method of electrotransformation and by detecting enzyme activity and the fermentation culture of BfC- 11 and BfC- 11B. The results showed that the activity of SerB and the yield of L- serine in BfC- 11B are higher than those in BfC - 11. It lays foundations for the research of construction of high L- serine produced gene - engineering strain.

关 键 词：磷酸丝氨酸转氨酶 克隆 表达 大肠杆菌 

分 类 号：TQ922.9[轻工技术与工程—发酵工程] Q78[生物学—分子生物学]