OsCOI1,水稻中一个受茉莉酸甲酯和脱落酸诱导表达的F-box家族基因  

OsCOI1, a Putative COI1 in Rice, Show MeJA and ABA Dependent Expression

作　　者：胡廷章[1] 王维平[1] 曹凯鸣[1] 王喜萍[1] 

机构地区：[1]复旦大学生命科学学院遗传工程国家重点实验室生物多样性与生态工程教育部重点实验室生物化学系

出　　处：《生物化学与生物物理进展》2006年第4期388-393,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(30270682,30170489);重庆市自然科学基金(8621)资助项目.

摘　　要：利用Blast检索、EST分析和RT-PCR,在水稻中分离到一个与拟南芥COI1同源的新基因,命名为OsCOI1.OsCOI1编码595个氨基酸.推测的OsCOI1编码蛋白有一个F-boxmotif和16个富含亮氨酸的重复序列,这与拟南芥COI1相似.OsCOI1在氨基酸水平上和COI1有很高的同源性(74%).经半定量RT-PCR法和RNA印迹分析,表明水稻中OsCOI1表达水平在经茉莉酸甲酯和脱落酸处理后呈明显变化,但不受水杨酸和乙烯的影响,说明OsCOI1可能在茉莉酸信号途径和脱落酸途径中具有特定功能.A novel gene, which was a homologue of Arabidopsis COI1 was isolated from rice （Oryza sativa L.） by RT-PCR and designated as OsCOI1. It encoded a protein of 595 amino acids. The similar F-box motif and 16 leucine-rich repeats were found in the deduced protein OsCOI1. OsCOI1 and COIl showed high homology （74%） at amino acid level. Semi-quantitative RT-PCR and Northern blot analysis demonstrated that the expression of OsCOI1 in rice varied obviously after treatment with MeJA and ABA but was not affected by SA and ET, suggesting that the specific function of OsCOI1 in JA signal pathway and related ABA pathway.

关 键 词：水稻 F-BOX MOTIF 半定量RT-PCR RNA印迹 茉莉酸信号途径 脱落酸途径 

分 类 号：Q5[生物学—生物化学] Q7