9-顺-维甲酸对人肺癌细胞株CD44、CD54表达的影响  

Effects of 9-cis-Retinoic Acid on the Expression of CD44 and CD54 in Lung Cancer Cell Lines

作　　者：张振江[1] 张蕊[2] 张世国[3] 陈景寒[1] 

机构地区：[1]山东大学山东省立医院胸外科,济南250021 [2]潍坊医学院麻醉药理学研究室 [3]济南军区总医院检验科

出　　处：《肿瘤防治研究》2006年第4期225-227,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(39770730)

摘　　要：目的探讨9-顺式维甲酸对肺癌细胞中粘附分子CD44、CD54表达的影响。方法应用流式细胞仪检测1umol/L9-cis-RA作用于肺癌细胞24、48、72h后CD44、CD54阳性标记率的变化。结果PG细胞株中CD44阳性标记率显著下降,CD54显著上升,这种影响9-cis-RA作用24小时后即显现,72小时仍存在。A549细胞株中CD54变化与PG细胞株类似,但CD44阳性标记率在各时相无明显变化。结论9-cis-RA可显著诱导肺癌细胞CD54或抑制CD44的表达。但在不同细胞,作用机制可能不尽相同。Objective To study the effect of 9-cis-retinoic acid （9-cis-RA） on the expression of both CD44 and CD54 in lung cancer cells. Methods 9-cis-RA （lumol/L） was used to treat lung cancer cells; Flow cytometry （FCM） was used to detect the positive marked rate of both CD44 and CD54 of three groups including blank control, DMSO control and 9-cis-RA groups. Results In PG cells, the marked rate of CD44 was significantly lower and the marked rate of CD54 was higher than that of the control groups; In A549 cells, similar effect on CD54 was seen. However, 9-cis-RA had no effect on the marked rate of CD44. Conclusion These findings suggests that 9-cis-RA exert its anti-metastatic function by virtue of either the induction of CD54 expression or the suppression of CD44 expression

关 键 词：抗肿瘤药物 9-顺-维甲酸 粘附分子CD44 CD54 肺癌细胞 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤]