粘虫中肠α-淀粉酶活性的敏感性研究  

Activity sensitivity of midgut α-amylase in larvae of the armyworm, Pseudaletia separata Walker (Lepidoptera: Noctuidae)

作　　者：黄青春[1] 卓军[1] 曹松[1] 钱旭红[1] 

机构地区：[1]华东理工大学药学院药物化工研究所,上海200237

出　　处：《昆虫学报》2006年第2期189-193,共5页Acta Entomologica Sinica

基　　金：国家重点基础研究发展规划项目(2003CB114402);国家自然科学基金项目(30400295)

摘　　要：研究了不同酶反应缓冲体系、pH值、氯离子浓度以及噁唑哒嗪对5龄2日粘虫PseudaletiaseparataWalker中肠α-淀粉酶活性的影响。结果表明,乙酸-乙酸钠缓冲体系(pH5.8)和磷酸氢二钠-磷酸二氢钠缓冲体系(pH8.0)有利于增强α-淀粉酶活性,比活力最高分别达到4.49和4.97。在乙酸-乙酸钠缓冲体系(pH5.8)中,5、10、20、40和80mmol/L氯离子浓度引起α-淀粉酶活性呈现先减弱后增强的变化规律,而在磷酸氢二钠-磷酸二氢钠缓冲体系(pH8.0)中仅呈现减弱的趋势。1.4mmol/L噁唑哒嗪对α-淀粉酶活性的抑制率可达70%,但抑制程度随着反应体系中蛋白含量的增加而逐渐降低。The effects of different enzymatic buffers, pH value, chloridion, and oxadiazolyl pyridazinone on the midgut α-amylase activity in 5th-instar 2nd-day larvae of Pseudaletia separata Walker were assayed. The results showed that sodium acetate buffer （pH 5.8） and phosphate buffer （pH 8.0） were most beneficial to α-amylase activity in their buffer series with different pH value, with the maximal activity as high as 4.49 and 4.97 U respectively. The activities against 5, 10, 20, 40, 80 mmol/L chloridion showed first decreasing and then increasing in sodium acetate buffer （pH 5.8 ）, whereas they showed only gradually decreasing in phosphate buffer （pH 8.0）. Inhibition of 1.4 mmol/L oxadiazolyl pyridazinone against α-amylase activity was as high as 70 percent, and thereafter gradually decreased with the increase of α-amylase protein.

关 键 词：粘虫 Α-淀粉酶 酶活性 PH值 氯离子 噁唑哒嗪 

分 类 号：Q965[生物学—昆虫学]