降解炼油废水菌株0297发酵条件的研究  

Fermentation Conditions of Bacterial Strain 0279 for Treating Refinery Wastewater

作　　者：严平[1] 朱永光[1] 吴斌[1] 李旭东[1] 廖银章[1] 

机构地区：[1]中国科学院成都生物所,成都610041

出　　处：《环境科学与技术》2006年第4期22-24,共3页Environmental Science & Technology

基　　金：国"家863"高技术研究发展专项基金资助项目(2002AA601150)

摘　　要：对筛选出的一株降解炼油废水的短状杆菌的发酵培养基组成(碳源,氮源及无机盐)和工艺条件(发酵温度,装液量)进行了单因素试验和正交优化试验,在不降低菌株降解性能的情况下,确定了发酵培养基组成:玉米粉0.4%,豆饼粉0.5%,MgSO4.7H2O0.4%,炼油废水50mL/100mL;最适发酵培养温度为35℃,最佳培养初始pH为5.5￣6.5,在250mL三角瓶中装40mL培养基,在145r/min摇床培养16h,发酵液中菌体浓度超过1010个/mL。Experiments were conducted to determine the optimum composition of fermentation medium for bacterial strain 0279, i.e., 0.4% for corn flour, 0.5% for bean powder, 0.4% MgSO4·7H2O; the relative parameters induding culture temperature 35 °C, pH range5-6.5 and 16h on a rotary shaker at 145rpm; and a concentration of 104cfu/mL was obtained in the ferment broth.

关 键 词：炼油废水 发酵 菌株 

分 类 号：X172[环境科学与工程—环境科学]