肉桂醛和柠檬醛对烟曲霉细胞膜中麦角甾醇生物合成的影响  

Effects of cinnamaldehyde and citral on ergosterol biosynthesis in Aspergillus fumigatus cell membrane

作　　者：谢小梅[1] 龙凯[1] 方建茹[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中草药》2006年第4期530-532,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30160099)

摘　　要：目的研究肉桂醛、柠檬醛对烟曲霉中麦角甾醇生物合成的影响。方法将烟曲霉接种在含有不同质量浓度药物的蔡氏固体培养基中,同时设不加药物为空白对照,置26.5℃恒温培养5 d,刮取平皿上菌苔,称质量、皂化、提取烟曲霉菌中的难皂化脂,高效液相色谱法测定其中的麦角甾醇。结果柠檬醛质量浓度为0.11μg/mL、肉桂醛质量浓度为0.16μg/mL作用于烟曲霉后,麦角甾醇的质量分数显著降低。结论肉桂醛和柠檬醛影响了烟曲霉中麦角甾醇的生物合成。Objective To investigate the effects of the active ingredients, cinnamaldehyde and citral,on ergosterol blosynthesis in Aspergillus fumigatus cell membrane, Methods Saponification and extraction of the nonsaponifiable lipids （NSLs） of A. fumigatus were carried out after 26.5 C for 5 d of incubation in Cai＇s solid medium with varied concentrations of cinnamaldehyde and citral compared with the control group winthont drugs. The contents of ergosterol in NSLs were determined by HPLC. Results After the treatment by cinnamaldehyde （0.16μg/mL） and citral （0.11μg/mL）, the contents of ergosterol in A. fumigatus cell membrane was obviously decreased. Conclusion Cinnamaldehyde and citral influence the ergosterol biosynthesis in A. fumigatus cell membrane.

关 键 词：肉桂醛 柠檬醛 烟曲霉 麦角甾醇 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]