MAGE-1与人HSP70融合基因真核表达载体的构建  

Construction of MAGE-1 and human HSP70 fusion gene eukaryotic expression vector

作　　者：陈广生[1] 隋延仿[1] 宋宏萍[1] 叶菁[1] 黄亚渝[1] 马加海[1] 张秀敏[1] 

机构地区：[1]第四军医大学附属西京医院病理科,陕西西安710032

出　　处：《现代肿瘤医学》2006年第5期516-518,共3页Journal of Modern Oncology

基　　金：国家自然科学基金(30271464);全军医药卫生科研基金(01Z084)重点资助项目

摘　　要：目的构建MAGE-1(melanomaantigen1)与人HSP70(heatshockprotein70)融合基因的真核表达载体pCDNA3.1-MAGE-1-HSP70,为肿瘤DNA疫苗研究奠定基础。方法利用PCR方法扩增人HSP70基因,经测序后连入真核表达载体pCDNA3.1,再通过PCR方法扩增MAGE-1基因,测序后插入HSP70基因的5'端,构建了MAGE-1与人HSP70融合基因表达载体pCDNA3.1-MAGE-1-HSP70。结果成功地扩增了人HSP70基因与MAGE-1基因,测序结果表明与GenBank公布的序列一致,成功地构建了pCDNA3.1-MAGE-1-HSP70真核表达载体。结论成功地构建MAGE-1与人HSP70融合基因的真核表达载体pCDNA3.1-MAGE-1-HSP70,为进一步DNA肿瘤疫苗研究提供了实验基础。Objective：To construct the MAGE - 1 and human HSP70 fusion gene eukaryotic expression vector Methods ：The HSP70 and MAGE - 1 genes were amplified by PCR. After sequencing, the two genes were cloned into the eukaryotic expression vector pCDNA3.1 to construct fusion gene expression plasmid pCDNA3.1 - MAGE - 1 - HSP70. Results：The HSP70 gene and MAGE - 1 gene were amplified successfully from plasmids, and their sequences were identical with that reported in GenBank. The fusion gene expression plasmid pCDNA3.1 - MAGE - 1 - HSP70 was successfully constructed. Conclusion ：The MAGE -1 and Hsp70 fusion gene eukaryotic expression vector pCDNA3.1 - MAGE - 1 - HSP70 was constructed, which prepared the materials for the research of DNA vaccine.

关 键 词：黑色素瘤抗原1 人热休克蛋白70 融合基因 

分 类 号：R730.3[医药卫生—肿瘤]