儿茶药材中儿茶素和表儿茶素的高效液相色谱法分析研究  

Studies on the Contents of Catechin and Epicatechin in Catechu by HPLC

作　　者：王淑敏[1] 李惠琳[1] 刘志强[1] 刘淑莹[1] 崔本连 

机构地区：[1]中国科学院长春应用化学研究所新药研究实验室 [2]吉林省通榆县中医院,137200

出　　处：《时珍国医国药》2006年第4期490-491,共2页Lishizhen Medicine and Materia Medica Research

基　　金：中国科学院知识创新工程重要方向项目资助(No.KGCX2-213-06)

摘　　要：目的 利用高效液相色谱法测定儿茶药材中儿茶素和表儿茶素的含量。方法 色谱柱填料为十八烷基键舍硅胶．流动相A：甲醇；流动相B：0．04mol/L柠檬酸-N，N-甲基甲酰胺（45：8，V／V）。A-B（15：85，V／V），流速1md／min。柱温：35℃，检测波长280nm。结果 儿茶素在0．484-4．84μg、表儿茶素在0．184—1．84iμg范围内呈良好的线性关系。两种成分的均回收率分别为98．11％和106．14％。结论 该方法简便、准确、分离效果好，无干扰，可用于儿茶药材质量评价。Objective To establish the quantitative analysis method of catechin and epicatechin in Catechu by HPLC. Methods The column was packed with 5μm Diamonsil C18 stationary phase. Mobile phase A： methanol; Mobile phase B： 0.04 mol/L citric acid-N, N-dimethylformamide（45： 8, V/V）. The mobile phase consisted of A-B（ 15：85, V/V）. The flow rate is 1ml/min. The column temperature was 35~C. The detection wavelength was set at 280 nm. Results The linear ranges of the two components are 0.484 -4.84 μg and 0. 184 - 1.84 μg respectively. The average recoveries of the two components are 98.11% and 106.14% respectively. Conclusion It is proved that the method is reliable, simple, and precise, which could be used for quality control of Catechu.

关 键 词：高效液相色谱 儿茶 儿茶素 表儿茶素 中药材 

分 类 号：R284.2[医药卫生—中药学] R282.71[医药卫生—中医学]