一株曲霉果糖转移酶的发酵条件及转果糖基活性  

Study on Fermentation Conditions for the Production of Fructosyltransferase and Transfructosylation from Aspergillus niger

作　　者：王立梅[1] 周惠明[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《食品与生物技术学报》2006年第2期52-55,共4页Journal of Food Science and Biotechnology

基　　金：江苏省科技计划项目(2001BA501A18)

摘　　要：对一株曲霉果糖转移酶菌株的产酶培养条件进行了研究。确定了最佳培养基组成：初始蔗糖质量浓度15～18g／dL，氮源为酵母膏，K2HPO4对果糖转移酶的产生具有明显的促进作用，添加0．2g／dLCMC能够使果糖转移酶活力提高到原来的1．3倍，在pH5．5，30℃条件下，果糖转移酶最高酶活力为30．42U／mL。HPLC分析结果表明，转糖基产物为总质量的55．8％。The optimal conditions for fructosyltransferase production from Aspergillus sp. were investigated. The initial concentration of sucrose was 15～18 g/dL. Yeast extract was the best nitrogen source. K2 HPO4 was effective in increasing enzyme production. The enzyme activity was increased 30% by addition of 0. 2 g/dL of CMC in the medium. The highest fructosyltrnsferase activity was 30.42 U/mL at pH 5. 5 and 30 ℃. Fructooligosaccharides was 55.8% of the total products.

关 键 词：果糖转移酶 转糖基 酶活力 生物量 

分 类 号：TQ925[轻工技术与工程—发酵工程]