临床化学上氨基酸、有机酸及核苷类的二维薄层色谱多相分离  

Simultaneous Separation of the Metabolins of Amino Acids,Organic Acids and Nucleosides in Clinical Chemistry by Two-Dimensional Thin-Layer Chromatography

作　　者：吴有光[1] 匡渤海[1] 龙飞[1] 

机构地区：[1]江西医学院

出　　处：《色谱》1996年第4期259-263,共5页Chinese Journal of Chromatography

摘　　要：在同一块薄层板上多相二维分离３０余种氨基酸、４０余种非挥发性有机酸和２０余种碱基。核苷类化合物。应用一对新的展开剂，用紫外灯－吖啶试剂－镉茚三酮试剂组合显色定位分析，改良了尿液非挥发性有机酸的溶剂萃取。尿液中此三类化合物提取物的多相二维色谱分离效果良好。Over 30 amino acids, 40 organic acids and 20 nucleosides and related compounds have been simultaneously separated by two-dimensional thin layer chromatography on a plate. The method of solvent extraction for the non-volatile organic acids in urine was improved. And the location of the separated compound spots was performed with a multiple procedures: Viewing the absorption spots in UV light (254nm)---colouration in acridine reagents for most organic acids and ninhydrin-cadmium acetate for amino acids.

关 键 词：二维薄层色谱法 氨基酸 有机酸 碱基核苷类 尿 

分 类 号：R446.12[医药卫生—诊断学] O658.64[医药卫生—临床医学]