Signal transduction of bombesin-induced circular smooth muscle cell contraction in cat esophagus  

作　　者：Sung-Uk Park Chang-Yell Shin Jung-Su Ryu Hyen-O La Sun-Young Park Hyun-Ju Song Young-Sil Min Dong-Seok Kim Uy-Dong Sohn 

机构地区：[1]Department of Pharmacology College of Pharmacy,Chung Ang University,Seoul 156-756,Republic of Korea [2]Department of Pharmacology College of Pharmacy,Chung Ang University,Seoul 156-756,Republic of Korea Research Laboratory,Dong-A Pharm. Co. Ltd,Yongin 449-905,Kyunggi-Do,Republic of Korea [3]Department of Pharmacology College of Medicine,The Catholic University of Korea,Seoul 137-701,Republic of Korea [4]Research Division for Human Life Sciences Seoul National University,28 Yongon-Dong,Chongno-Gu,Seoul 110-744,Republic of Korea

出　　处：《World Journal of Gastroenterology》2006年第14期2259-2263,共5页世界胃肠病学杂志（英文版）

摘　　要：AIM： To investigate the mechanism of bombesin-induced circular smooth muscle cell contraction in cat esophagus. METHODS： Specific G protein or phospholipase C involved in cat esophagus contraction was identified, muscle cells were permeabilized with saponin. After per- meabilization of muscle cells, the Gi3 antibody inhibited bombesin-induced smooth muscle cell contraction. RESULTS： Incubation of permeabilized circular muscle cells with PLC-β3 antibody could inhibit bombesin-induced contraction. H-7, chelerythrine （PKC inhibitor） and genistein （protein tyrosine kinase inhibitor） inhibited bombesin-induced contraction, but DAG kinase inhibitor, R59949, could not inhibit it. To examine which mitogen-activated protein kinase （MAPK） was involved in bombesin-induced contTaction, the specific MAPK inhibitors （MEK inhibitor, PD98059 and p38 MAPK inhibitor, SB202190） were used. Preincubation of PD98059 blocked the contraction induced by bombesin in a concentration-dependent manner. However, SB202190 had no effects on contraction. CONCLUSION： Bombesin-induced circular muscle cell contraction in cat esophagus is mediated via a PKC or a PTK-dependent pathway or p44/p42 HAPK pathway.瞄准：在猫食管调查导致 bombesin 的圆形的平滑肌房间收缩的机制。方法：涉及猫食管收缩的特定的 G 蛋白质或磷脂酶 C 被识别，肌细胞是有皂甙的 permeabilized。在肌细胞的 permeabilization 以后， Gi3 抗体禁止了导致 bombesin 的平滑肌房间收缩。结果：有 PLC-beta3 抗体的 permeabilized 通报肌细胞的孵化能禁止导致 bombesin 的收缩。H-7，白屈菜红(PKC 禁止者) 和 genistein (蛋白质酷氨酸激酶禁止者) 禁止了导致 bombesin 的收缩，而是 DAG 激酶禁止者， R59949，不能禁止它。检验激活 mitogen 的蛋白质激酶(MAPK ) 哪个涉及导致 bombesin 的收缩，特定的 MAPK 禁止者(MEK 禁止者， PD98059 和 p38 MAPK 禁止者， SB202190 ) 被使用。PD98059 的 Preincubation 堵住了 bombesin 以一种集中依赖者方式导致的收缩。然而， SB202190 没在收缩上有效果。结论：在猫食管的导致 Bombesin 的圆形的肌肉房间收缩是经由 PKC 或一条 PTK 依赖的小径或 p44/p42 MAPK 小径的 madiated。

关 键 词：BOMBESIN G protein Phospholipase C Protein kinase C Protein tyrosine kinase MAP Kinase Cell contraction 

分 类 号：R571[医药卫生—消化系统]