3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮诱导人胚胎肝细胞ras基因突变  

Mutation of ras Genes Induced by 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H] -furanone in Human Fetal Hepatocytes

作　　者：周利红[1] 刘爱林[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系和教育部环境与健康重点实验室,湖北武汉430030

出　　处：《癌变．畸变．突变》2006年第3期171-174,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(No.30170794)

摘　　要：背景与目的:研究饮水氯化消毒副产物3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮(3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone,MX)对体外培养的人胚胎肝细胞(L-02细胞)ras基因突变的诱导。材料与方法:MX染毒剂量为300μmol/L,以二甲基亚砜(DMSO)做溶剂对照,将L-02细胞连续染毒培养12d后,收获细胞提取基因组DNA,应用PCR-克隆测序法检测ras基因(K-ras、H-ras、N-ras)12、13、61密码子是否存在突变。结果:MX染毒组H-ras基因57密码子的GAT置换成GGT,未检测到K-ras、N-ras及H-ras12、13、61密码子突变,DMSO溶剂对照组相应的ras基因目的片段均未检测到突变。结论:MX可能诱导L-02细胞ras基因突变。BACKGROUND ＆ Albl： To study the mutation of ras genes induced by 3-chloro-4-（diehloromethyl）-5-hydroxy-2[5H] -furanone （MX） in human fetal hepatocytes （L-02） in vitro. MATERIAL AND METHODS： L-02 cells were treated consecutively with 300 μmol/ L of MX for 12 days and genome DNA was extracted after cell harvesting.Dimethyl sulfoxide （DMSO） was used as solvent control. Ras genes（K-ras, H-ras, N-ras） sequences including 12, 13 and 61 codons were tested using the PCR-clone and sequencing with the genome DNA from the cells. RESULTS： H-ras gene 57 codon substitution from GAT to GGT in the MX-treated group was found, but 12, 13, 61 codons of K-ras,H-ras, N-ras were the same as the normal sequence. There was no mutation in the solvent control group. CONCLUSION：MX probably induced ras gene mutation of L-02 cells.

关 键 词：3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮 RAS基因 基因突变 

分 类 号：R394.6[医药卫生—医学遗传学] R994.6[医药卫生—基础医学]