杏仁对吸烟导致DNA氧化损伤的保护作用  

The Protective Effect of Almond on DNA Damage Induced by Cigarette Smoke

作　　者：张文众[1] 张晓鹏[1] 贾旭东[1] 黄光伟 刘兆平[1] 宋雁[1] 刘泽嵚[1] 崔文明[1] 张馨[1] 王伟[1] 李宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050 [2]美国加州杏仁协会,美国加州95354

出　　处：《癌变．畸变．突变》2006年第3期208-210,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:研究美国加州杏仁对吸烟诱导的DNA氧化损伤是否具有保护作用。材料与方法:选取30个吸烟的志愿者,随机分3组(每组10人),每天分别给予0,84和168g的杏仁,共28d;试验期间各组对象膳食和吸烟量相同,在实验开始前和试验结束后分别收集新鲜尿液测定柯替宁,采静脉血做彗星实验,收集24h尿测定8-OH-dG。结果:彗星实验显示,168g/d剂量组DNA损伤程度比对照组和试验前均显著降低(P<0.05);8-OH-dG测定结果显示,84g/d和168g/d剂量组的8-OH-dG水平明显低于试验前和对照组(P<0.05)。结论:食用美国加州杏仁可能具有降低吸烟人群DNA氧化损伤的作用。BACKGROUND ＆ AIM：Exposure of cigarette smoke （CS） can increase oxidative damage, this research aimed to study the protective effect of supplementation of almond on DNA damage in smokers.MATERIAL AND METHOBS：Thirty smokers were recruited into our study, and were divided into three groups（n = 10） which received almond 0 g/d,84 g/d and 168 g/d respectively, and the study period was for 28 days. In the beginning and the end of the study,eotinine and 8-hydroxy- 2＇deoxyguanosine （8-OH-dG） levels in urine were analysed, and comet assay of peripheral blood lymphocytes was conducted, RESULTS： In the end of the study , compared with control group and baseline, a significant decrease of oliveive tail moment and tail/head length in comet assay in 168 g/d group was observed （P〈0.05）. The 8-OH-dG level decreased significantly in 84 g/d and 168 g/d groups（P 〈0.05） . CONCLUSION： The results suggested that supplementation of almond might reduce DNA damage in smokers.

关 键 词：美国加州杏仁 吸烟 彗星试验 8-羟基脱氧鸟苷 

分 类 号：R361[医药卫生—病理学] R163[医药卫生—基础医学]