环境内毒素相关新基因的克隆测序及生物信息学分析  

Cloning, Sequencing and Bioinformatics Analysis of Environmental Endotoxin Related New Gene

作　　者：杜可军[1] 常文辉[2] 侯立朝[3] 刘承利[3] 宋庆贺[4] 陈苏民[4] 柴玉波[4] 陈景元[1] 

机构地区：[1]第四军医大学劳动与环境卫生学教研室,陕西西安710032 [2]陕西省疾病预防控制中心病毒室,陕西西安710032 [3]第四军医大学西京医院麻醉科,陕西西安710032 [4]第四军医大学生物化学与分子生物学教研室,陕西西安710032

出　　处：《环境与健康杂志》2006年第3期211-213,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30170361;30400413;30571586);第四军医大学博士后基金资助项目(00001149)

摘　　要：目的克隆表达环境内毒素相关新基因——人lrg基因(简称Hlrg),并进行生物信息学分析。方法采用RT-PCR扩增Hlrg基因,对扩增产物进行序列的测定,利用Internet和GenBank数据库对测序结果正确的Hlrg基因进行生物信息学分析。结果克隆表达了Hlrg基因,测序结果正确;生物信息学分析表明,该序列编码186个氨基酸,GenBank中不含有同源序列。预测相对分子量为21×103,该序列包含有亮氨酸拉链结构,可能具有重要的功能。结论成功扩增、克隆表达了Hlrg基因,对其功能进行了初步预测,为进一步的功能研究打下基础。Objective To amplify and clone human lrg and to predict its function by bioinformatics analysis. Methods The human lrg was amplified by RT-PCR, then identified by sequencing. Function of human lrg was predicted by bioinformatics analysis with Internet and GenBank database. Results The human lrg was amplified and sequenced correctly. Leucine zipper was found in the human lrg series that may have an important function. Conclusion The human lrg gene has been successfully subeloned and its function has been predicted. The result of the present paper will provide data and evidences for the further study on function of human lrg.

关 键 词：基因 Hlrg基因 克隆 分子 序列分析 

分 类 号：R994.6[医药卫生—毒理学]