中国林蛙输卵管上皮细胞的培养及其生物学特性研究  

Culture of Rana temporaria chensinensis David Oviductal Epithelial Cell andI tsB iologicalC haracteristics

作　　者：郑鑫[1] 刘景圣[2] 夏清娟[1] 昌倩宇[2] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]吉林农业大学食品工程学院,吉林长春130118

出　　处：《食品科学》2006年第5期118-121,共4页Food Science

基　　金：国家自然基金资助项目(30471265)

摘　　要：本实验对中国林蛙(RanatemporariachensinesisDavid)的输卵管上皮细胞,成功地进行了原代及传代体外培养,并对输卵管上皮细胞的生物学特性进行观察、鉴定研究,建立了一种稳定的林蛙输卵管上皮细胞体外培养方法,为进一步研究其生物活性物质奠定了实验基础。Rana temporaria chensinesis David oviductal epithelial cell was primary cultured and subcultured in vitro, these cells were identified epithelial cell by using immuno-reactivity. The growth and morphous of the primary cells and subculture cells were observed by inverted oplic microscope. The primary, the third and fifth subculture cells were cultured and counted, respectively. Multiplication capacity of the third subculture cells is stronger than the primary cells and the fifth subculture cells. A stable culture in vitro method of Rana temporaria chensinesis David oviductal epithelial cell was established. This culture method is the base for the further study of its biological active substances.

关 键 词：中国林蛙 输卵管上皮细胞 细胞培养 体外培养 生物学特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]