高压液相色谱法测定金丝小枣环磷酸腺苷的含量  

Determination of Cyclic Adenosine-3’,5’-monophosphate in Golden Silk Jujube by High Performance Chromatography

作　　者：史红梅[1] 尹卓容[1] 蒋文强[1] 

机构地区：[1]山东轻工业学院,山东济南250100

出　　处：《食品科学》2006年第5期216-218,共3页Food Science

摘　　要：建立了用高压液相色谱法测定枣汁中环磷酸腺苷的方法。用Shim-PackVP-ODS色谱柱,流动相为甲醇-0.1mol/L磷酸二氢钾缓冲液(30:70,V/V),检测波长260nm,流速0.6ml/min,回收率101.5%。该法简便,灵敏,重现性好,可以快速测定含环磷酸腺苷的溶液及其制剂的含量。A high performance chromatographic method was developed for the determination of cyclic adenosine-3 ＇,5＇ - monophosphate in golden silk jujube. The separation was obtained using a Shim-Pack VP-ODS column with a mobile phase of methanol-0.1mol/L phosphate buffer （30：70, V/V）, and detected at 260nm. The method is simple, sensitive, reproducible and it can rapidly determine the cAMP solution and its content.

关 键 词：环磷酸腺苷 高压液相色谱 金丝小枣 

分 类 号：O652.63[理学—分析化学]