Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation  

作　　者：LI Zi-jian LIU Ning LIU Zhi-qiang LIU Shu-ying HAN Qi-de ZHANG You-yi 

机构地区：[1]Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory ofMolecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, P. R. China [2]Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

出　　处：《Chemical Research in Chinese Universities》2006年第3期319-323,共5页高等学校化学研究（英文版）

基　　金：SupportedbytheNationalKeyBasicResearchProgramofChina(No.G2000056906),theNationalNaturalScienceFoundationofChina(Nos.30400552and20273067)andtheResearchFundfortheDoctoralProgramofHigherEducation(No.20030001014).

摘　　要：β-Adrenoceptors（β-ARs） play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through which β-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to iseproterenol （ISO）. A modified method, ＂Mirror Images in One Gel＂, was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 ± 70 spots were detected and about 1191± 54 spots were matched, with an average matching rate of 92. 9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting（PMF） data obtained vht MALDI-MS.β-Adrenoceptors（β-ARs） play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through which β-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to iseproterenol （ISO）. A modified method, ＂Mirror Images in One Gel＂, was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 ± 70 spots were detected and about 1191± 54 spots were matched, with an average matching rate of 92. 9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting（PMF） data obtained vht MALDI-MS.

关 键 词：PROTEOME Two-dimensional electrophoresis（2-DE） MALDI-MS Β-ADRENOCEPTOR CARDIOMYOCYTE 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]