内皮素反义基因治疗门静脉高压症的研究  

Effects of endothelin-1 on portal hypertension induced by endotoxin in vivo

作　　者：毕向军[1] 陈旻湖[2] 陈文红[2] 陈为[2] 王锦辉[2] 朱森林[2] 

机构地区：[1]广州军区广州总医院干部内二科,广州市510010 [2]中山大学第一附属医院消化内科,广州市510010

出　　处：《中华肝胆外科杂志》2006年第4期250-252,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：本研究受2002年广东省自然科学基金重点项目资助(21889)

摘　　要：目的研究内皮素-1反义寡核苷酸(ET1-ASON)在内毒素(LPS)致门静脉高压中的作用。方法应用 ET1-ASON 预处理后将 LPS 以1 mg/kg 体重腹腔注射大鼠,以正义、错义 ET1-ASON 和生理盐水作为对照,4 h 后检测大鼠 PVF、PVP、PVR 和 IVCP应用放射免疫法、RT-PCR 检测各组门静脉血和肝脏的 ET1表达情况。结果 ET1-ASON 处理组大鼠门静脉血浆和肝脏组织ET1表达减低;PVR 和 PVP 较对照组明显降低,PVF 无明显改变。结论 ET1-ASON 在内毒素致门脉高压中主要通过降低 PVR 起作用。Objective To investigate the effects of endothelin-1 （ET1） on portal hypertension induced by endotoxin. Methods The rats were intraperitoneally injected with different doses of LPS. PVP, IVCP, PVF and PVR were recorded 4 h after the injection. ET1 anti-sense oligonucleotide and cation lipofectamine were mixed and then injected into the portal vein of rats. Twelve hours later, 1 mg/kg LPS was injected and PVP, IVCP and PVF were recorded again. The liver and portal blood samples were used to detect ET1 by radioimmunoassay. ET1 mRNA of the liver tissue was detected by RT-PCR. Results The PVF and PVP in the rats were obviously increased with the dose of LPS. PVR decreased and the decrease was not related to the dose of LPS. ET1 antisense oligonucleotide partially counteracted the effects of LPS on portal hemodynamics by decreasing PVR and PVP. LPS prompted the expression of ET1 in liver tissue and serum of the rats. Conclusions ET1 plays an important role in portal hypertension induced by endotoxin through increasing PVR. ET1 antisense oligonucleotide can partially inhibit portal hypertension induced by LPS.

关 键 词：高血压 门静脉 内毒素 内皮素-1 反义寡核苷酸 

分 类 号：R575.2[医药卫生—消化系统]